hPop5, a Protein Subunit of the Human RNase MRP and RNase P Endoribonucleases*
hPop5, a Protein Subunit of the Human RNase MRP and RNase P Endoribonucleases*
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hPop5，人类 RNase MRP 和 RNase P 核糖核酸内切酶的蛋白质亚基*
DOI：
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发表时间：
2001
影响因子：
4.8
通讯作者：
G. Pruijn
中科院分区：
文献类型：
--
作者：
H. van Eenennaam;D. Lugtenberg;Judith Vogelzangs;W. V. van Venrooij;G. Pruijn
RNase MRP和RNase P颗粒都起核糖核酸内切酶的作用。RNase MRP参与了核糖体rRNA的加工，而RNase P则参与了前tRNA的加工。两种核糖核蛋白颗粒都有一种RNA成分，可以折叠成类似的二级结构，并共享几种蛋白质成分。我们已经确定了人类，大鼠，小鼠，奶牛，和果蝇同源的Pop 5 p蛋白亚基的酵母RNase MRP和RNase P复合物。人Pop 5 cDNA编码163个氨基酸的蛋白质，预测分子量为18.8 kDa。针对重组hPop 5的多克隆抗体鉴定了HeLa细胞中的19-kDa多肽，并显示hPop 5与RNase MRP和RNase P两者相关。使用亲和纯化的抗hPop 5抗体，我们证明内源性hPop 5蛋白定位于细胞核中并在核仁中积累，这与它与RNaseMRP和RNaseP的结合一致。催化活性RNaseP从HeLa细胞中部分纯化，并且hPop 5显示与它结合。最后，hPop 5的进化上保守的酸性C-末端尾似乎既不是复合物形成所需的，也不是RNaseP活性所需的。
The RNase MRP and RNase P particles both function as endoribonucleases. RNase MRP has been implicated in the processing of precursor-rRNA, whereas RNase P has been shown to function in the processing of pre-tRNA. Both ribonucleoprotein particles have an RNA component that can be folded into a similar secondary structure and share several protein components. We have identified human, rat, mouse, cow, and Drosophila homologues of the Pop5p protein subunit of the yeast RNase MRP and RNase P complexes. The human Pop5 cDNA encodes a protein of 163 amino acids with a predicted molecular mass of 18.8 kDa. Polyclonal antibodies raised against recombinant hPop5 identified a 19-kDa polypeptide in HeLa cells and showed that hPop5 is associated with both RNase MRP and RNase P. Using affinity-purified anti-hPop5 antibodies, we demonstrated that the endogenous hPop5 protein is localized in the nucleus and accumulates in the nucleolus, which is consistent with its association with RNase MRP and RNase P. Catalytically active RNase P was partially purified from HeLa cells, and hPop5 was shown to be associated with it. Finally, the evolutionarily conserved acidic C-terminal tail of hPop5 appeared to be required neither for complex formation nor for RNase P activity.
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DOI：
10.1073/pnas.95.12.6716
发表时间：
1998-06
影响因子：
11.1
作者：
V. Štolc;A. Katz;S. Altman
通讯作者：
V. Štolc;A. Katz;S. Altman
影响因子：
56.9
作者：
GOLD, HA;TOPPER, JN;CRAFT, J
通讯作者：
CRAFT, J
影响因子：
10.5
作者：
Chamberlain, JR;Lee, Y;Engelke, DR
通讯作者：
Engelke, DR
影响因子：
56.9
作者：
CHANG, DD;CLAYTON, DA
通讯作者：
CLAYTON, DA
使用硬皮病患者的自身抗体检测核仁 7-2 核糖核蛋白和细胞质 8-2 核糖核蛋白。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Reddy,R;Tan,EM;Henning,D;Nohga,K;Busch,H
通讯作者：
Busch,H