An ex vivo study of the correlation between acoustic emission and microvascular damage.
An ex vivo study of the correlation between acoustic emission and microvascular damage.
复制标题
DOI：
10.1016/j.ultrasmedbio.2009.04.013
复制
发表时间：
2009-09
影响因子：
2.9
通讯作者：
Miller, Douglas L.
中科院分区：
文献类型：
--
作者：
Samuel, Stanley;Cooper, Michol A.;Bull, Joseph L.;Fowlkes, J. Brian;Miller, Douglas L.
本研究的目的是对声发射与组织损伤之间的相关性进行离体检查。对雄性 Wistar 大鼠的提睾肌进行活体显微镜检查并结合超声波照射。 Definity® 微泡借助注射泵通过尾静脉静脉注射（80 μL.kg-1.min-1 输注速度）。对于脉冲重复频率 (PRF) 研究，在提睾肌的四个位置进行暴露，PRF 分别为 1000、500、100 和 10 Hz（每只大鼠每个 PRF 一个位置）。 100 脉冲曝光是在 2 MPa 峰值稀疏压力 (Pr)、2.25 MHz 频率和 46 个周期脉冲下进行的。对于压力幅度阈值研究，在 0.5 MPa 至 2 MPa 的各种峰值稀疏压力、频率 2.25 MHz 和 PRF 100 Hz 下进行 100 脉冲暴露（46 个周期脉冲）。显微照片是在曝光前和曝光后 2 分钟拍摄的。在脉冲到脉冲的基础上，10 Hz 声发射比其他 PRF（1000、500 和 100 Hz）的声发射要高得多且更持久（p < 0.05）。以红细胞 (RBC) 外渗面积衡量的损伤在 10 Hz PRF 下也显着高于 1000、500 和 100 Hz (p < 0.01)。声发射与组织损伤的相关性显示出随着相对积分功率谱累积函数（CRIPS；R2 = 0.75）的增加，损伤增加的趋势。 Pr ≤ 0.85 MPa 时不存在可见损坏。然而，在 Pr ≥ 1.0 MPa 时观察到损坏，并且损坏随着声压的增加而增加。
The objective of this study was to conduct an ex vivo examination of correlation between acoustic emission and tissue damage. Intravital microscopy was employed in conjunction with ultrasound exposure in cremaster muscle of male Wistar rats. Definity® microbubbles were administered intravenously through the tail vein (80 μL.kg-1.min-1infusion rate) with the aid of a syringe pump. For the pulse repetition frequency (PRF) study, exposures were performed at four locations of the cremaster at a PRF of 1000, 500, 100 and 10 Hz (one location per PRF per rat). The 100-pulse exposures were implemented at a peak rarefactional pressure (Pr) of 2 MPa, frequency of 2.25 MHz with 46 cycle pulses. For the pressure amplitude threshold study, 100-pulse exposures (46 cycle pulses) were conducted at various peak rarefactional pressures from 0.5 MPa to 2 MPa at a frequency of 2.25 MHz and PRF of 100 Hz. Photomicrographs were captured before and 2-minutes post exposure. On a pulse-to-pulse basis, the 10 Hz acoustic emission was considerably higher and more sustained than those at other PRFs (1000, 500, and 100 Hz) (p < 0.05). Damage, measured as area of extravasation of red blood cells (RBC's), was also significantly higher at 10 Hz PRF than at 1000, 500, and 100 Hz (p < 0.01). The correlation of acoustic emission to tissue damage showed a trend of increasing damage with increasing cumulative function of the relative integrated power spectrum (CRIPS; R2 = 0.75). No visible damage was present at Pr ≤ 0.85 MPa. Damage, however, was observed at Pr ≥ 1.0 MPa, and it increased with increasing acoustic pressure.
登录
查看更多内容
影响因子：
2.9
作者：
Everbach, EC;Makin, IRS;Meltzer, RS
通讯作者：
Meltzer, RS
影响因子：
2.9
作者：
Li, P;Armstrong, WF;Miller, DL
通讯作者：
Miller, DL
影响因子：
2.9
作者：
Maruvada, S;Hynynen, K
通讯作者：
Hynynen, K
影响因子：
2.9
作者：
Miller, Douglas L.
通讯作者：
Miller, Douglas L.
影响因子：
2.9
作者：
Samuel, S;Miller, DL;Fowlkes, JB
通讯作者：
Fowlkes, JB