Inflammatory response to nano- and microstructured hydroxyapatite.
Inflammatory response to nano- and microstructured hydroxyapatite.
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DOI：
10.1371/journal.pone.0120381
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ott MK
中科院分区：
文献类型：
--
作者：
Mestres G;Espanol M;Xia W;Persson C;Ginebra MP;Ott MK
与生物材料接触后白细胞的增殖和活化在炎症反应的程度中起着至关重要的作用，这可能决定植入生物材料的临床失败或成功。本研究的目的是评估纳米和微米结构仿生羟基磷灰石基材是否可以影响巨噬细胞样细胞的生长和活化。对具有不同晶体形态的羟基磷灰石基材进行了评估，该基材由板状和针状晶体的缠结网络组成。在材料表面（直接接触）以及提取物（即经材料修饰的介质（间接接触））中评估巨噬细胞增殖。此外，还研究了用钙离子和/或蛋白质补充提取物的效果。通过量化活性氧的释放和形态学观察来评估基质上的巨噬细胞活化。结果表明，基质微观结构的差异在巨噬细胞的激活中起着重要作用，因为在板状晶体基质上培养巨噬细胞后，活性氧的释放量更高，而在针状基质上几乎不存在活性氧的释放。然而，巨噬细胞增殖的差异归因于不同的离子交换和蛋白质从基质的吸附/保留，而不是材料的质地。
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate’s microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.
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影响因子：
3.2
作者：
Albrecht, Catrin;Scherbart, Agnes M.;Scheel, Julia
通讯作者：
Scheel, Julia
β-磷酸三钙通过 BMP2 信号通路刺激巨噬细胞诱导骨髓 MSC 成骨分化
影响因子：
14
作者：
Chen, Zetao;Wu, Chengtie;Xiao, Yin
通讯作者：
Xiao, Yin
DOI：
10.1073/pnas.0306141101
发表时间：
2004-04-06
影响因子：
11.1
作者：
Dvorak, MM;Siddiqua, A;Riccardi, D
通讯作者：
Riccardi, D
DOI：
10.1016/s1389-0344(02)00019-9
发表时间：
2002-08-01
期刊：
BIOMOLECULAR ENGINEERING
影响因子：
--
作者：
Kirkpatrick, CJ;Krump-Konvalinkova, V;Peters, K
通讯作者：
Peters, K
影响因子：
9.7
作者：
Gustavsson, J.;Ginebra, M. P.;Planell, J.
通讯作者：
Planell, J.