The dual origin of the peripheral olfactory system: placode and neural crest.
The dual origin of the peripheral olfactory system: placode and neural crest.
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DOI：
10.1186/1756-6606-4-34
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发表时间：
2011-09-23
期刊：
影响因子：
3.6
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Katoh H;Shibata S;Fukuda K;Sato M;Satoh E;Nagoshi N;Minematsu T;Matsuzaki Y;Akazawa C;Toyama Y;Nakamura M;Okano H
嗅觉上皮(OE)具有独特的持续神经发生能力，在嗅鞘细胞(OECs)的帮助下将轴突延伸至嗅球。OE和OECs被认为仅由嗅觉平台发育而来，而神经脊(NC)细胞被认为只贡献了嗅觉系统的基本结构元素。为了进一步阐明NC细胞在嗅觉发育中的作用，我们检测了永久性表达GFP的NC细胞及其后代转基因小鼠WNT1-CRE/FLOXED-EGFP和P0-CRE/FLOXED-EGFP的嗅觉系统，并在鸡胚胎中进行了转座子介导的细胞谱系追踪研究。对这些转基因小鼠的检查发现，OE中有GFP阳性细胞，表明NC来源的细胞产生了OE细胞，其形态和抗原特性与胎盘来源的细胞相同。嗅鞘细胞也呈GFP阳性，证实其为NC来源。在鸡胚胎中进行的细胞谱系追踪研究证实了NC细胞迁移到OE中。此外，从分离的嗅黏膜细胞培养的球体显示出自我更新和三线分化能力(神经元、神经胶质细胞和肌成纤维细胞)，表明嗅黏膜中存在NC前体细胞。我们的数据表明，NC在嗅觉系统的发育中发挥着比先前认为的更大的作用，并表明NC来源的细胞可能部分地对OE具有非凡的神经发生和再生能力负责。
The olfactory epithelium (OE) has a unique capacity for continuous neurogenesis, extending axons to the olfactory bulb with the assistance of olfactory ensheathing cells (OECs). The OE and OECs have been believed to develop solely from the olfactory placode, while the neural crest (NC) cells have been believed to contribute only the underlying structural elements of the olfactory system. In order to further elucidate the role of NC cells in olfactory development, we examined the olfactory system in the transgenic mice Wnt1-Cre/Floxed-EGFP and P0-Cre/Floxed-EGFP, in which migrating NC cells and its descendents permanently express GFP, and conducted transposon-mediated cell lineage tracing studies in chick embryos. Examination of these transgenic mice revealed GFP-positive cells in the OE, demonstrating that NC-derived cells give rise to OE cells with morphologic and antigenic properties identical to placode-derived cells. OECs were also positive for GFP, confirming their NC origin. Cell lineage tracing studies performed in chick embryos confirmed the migration of NC cells into the OE. Furthermore, spheres cultured from the dissociated cells of the olfactory mucosa demonstrated self-renewal and trilineage differentiation capacities (neurons, glial cells, and myofibroblasts), demonstrating the presence of NC progenitors in the olfactory mucosa. Our data demonstrates that the NC plays a larger role in the development of the olfactory system than previously believed, and suggests that NC-derived cells may in part be responsible for the remarkable capacity of the OE for neurogenesis and regeneration.
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影响因子：
4.2
作者：
CHUAH, MI;AU, C
通讯作者：
AU, C
DOI：
10.1073/pnas.0501263102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Amoh, Y;Li, L;Hoffman, RM
通讯作者：
Hoffman, RM
影响因子：
2.5
作者：
Harada, Hidekiyo;Takahashi, Yoshiko;Nakamura, Harukazu
通讯作者：
Nakamura, Harukazu
影响因子：
2.7
作者：
COULY, GF;LEDOUARIN, NM
通讯作者：
LEDOUARIN, NM
影响因子：
16.2
作者：
BHATTACHARYYA, A;FRANK, E;BRACKENBURY, R
通讯作者：
BRACKENBURY, R