Detection and Isolation of Rickettsia tillamookensis (Rickettsiales: Rickettsiaceae) From Ixodes pacificus (Acari: Ixodidae) From Multiple Regions of California
Detection and Isolation of Rickettsia tillamookensis (Rickettsiales: Rickettsiaceae) From Ixodes pacificus (Acari: Ixodidae) From Multiple Regions of California
复制标题
加州多个地区的太平洋硬蜱（螨虫：蜱科）中检测和分离蒂拉莫克立克次体（立克次体目：立克次体科）
DOI：
10.1093/jme/tjac038
复制
发表时间：
2022
影响因子：
2.1
通讯作者：
Zhong, Jianmin
中科院分区：
文献类型：
--
作者：
Paddock, Christopher D;Slater, Kimetha;Swei, Andrea;Zambrano, Maria L;Kleinjan, Joyce E;Padgett, Kerry A;Saunders, Megan E;Andrews, Elizabeth S;Trent, Erin;Zhong, Jianmin
西部黑腿蜱（太平洋伊蚊）是美国西部最常见的咬人蜱种，也是至少三种已知的人类细菌病原体的主要媒介。一种潜在致病性立克次体在1978年首次被描述，最近被定性为一种新的过渡性病原体，被命名为立克次体，也存在于人群中。虽然这种药剂的分布和频率尚不清楚。我们评估了348个寻找宿主的DNA提取物。从加利福尼亚5个县的9个地点收集的太平洋若虫。采用实时荧光定量PCR技术对13个县24个地点的成虫进行检测。tillamookensis。DNA雌花。在北加州11个县的10只若虫（2.9%）（95% CI: 1.6 ~ 5.2%）和17只成虫（1.9%）（95% CI: 1.2 ~ 3.0%）中检测到tillamookensis。虽然地点特异性感染率差异很大，但按阶段、性别、生境类型或地理区域汇总时，感染频率始终较低。4个新分离株。在内华达州阿拉米达县和约洛县收集的成年蜱的Vero E6细胞中培养tillamookensi。40多年前被血清分型为“tillamook样”菌株的4株历史分离株从液氮中长期储存中复活，随后通过分子方法确认为r分离株。tillamookensis。r的潜在公共卫生影响。蒂拉莫肯斯需要进一步调查。
The western black-legged tick (Ixodes pacificus) is the most frequently identified human-biting tick species in the western United States and the principal vector of at least three recognized bacterial pathogens of humans. A potentially pathogenicRickettsiaspecies, first described in 1978 and recently characterized as a novel transitional group agent designated asRickettsia tillamookensis, also exists among populations ofI. pacificus, although the distribution and frequency of this agent are poorly known. We evaluated DNA extracts from 348 host-seekingI. pacificusnymphs collected from 9 locations in five California counties, and from 916I. pacificusadults collected from 24 locations in 13 counties, by using a real-time PCR designed specifically to detect DNA ofR. tillamookensis.DNA ofR. tillamookensiswas detected in 10 (2.9%) nymphs (95% CI: 1.6–5.2%) and 17 (1.9%) adults (95% CI: 1.2–3.0%) from 11 counties of northern California. Although site-specific infection rates varied greatly, frequencies of infection remained consistently low when aggregated by stage, sex, habitat type, or geographical region. Four novel isolates ofR. tillamookensiswere cultivated in Vero E6 cells from individual adult ticks collected from Alameda, Nevada, and Yolo counties. Four historical isolates, serotyped previously as ‘Tillamook-like’ strains over 40 yr ago, were revived from long-term storage in liquid nitrogen and confirmed subsequently by molecular methods as isolates ofR. tillamookensis. The potential public health impact ofR. tillamookensisrequires further investigation.
登录
查看更多内容
影响因子：
3.3
作者：
R. Lane;R. Emmons;D. Dondero;B. C. Nelson
通讯作者：
B. C. Nelson
影响因子：
2.4
作者：
ARTHUR, DR;SNOW, KR
通讯作者：
SNOW, KR
混合传播模式促进了新出现的蜱传病原体的持续存在
影响因子：
2.7
作者：
Sambado, Samantha;Salomon, Jordan;Crews, Arielle;Swei, Andrea
通讯作者：
Swei, Andrea
墨西哥硬蜱属（螨虫：硬蜱科）：成虫识别码、诊断、宿主和分布
DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
C. Guzmán;R. Robbins
通讯作者：
R. Robbins
DOI：
10.1099/00207713-45-1-23
发表时间：
1995-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
TULLY, JG;ROSE, DL;WHITCOMB, RF
通讯作者：
WHITCOMB, RF