Real-time bioluminescence imaging of mixed mycobacterial infections.
Real-time bioluminescence imaging of mixed mycobacterial infections.
复制标题
混合分枝杆菌感染的实时生物发光成像。
DOI：
10.1371/journal.pone.0108341
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cirillo JD
中科院分区：
文献类型：
--
作者：
Chang M;Anttonen KP;Cirillo SL;Francis KP;Cirillo JD
细菌感染过程的分子分析通常涉及等基因突变体的构建，然后可以将其与动物模型中的野生型进行比较。由于动物之间的差异和需要在特定时间点牺牲个别动物，发病机制和抗菌研究变得复杂。活体动物成像允许实时分析感染，而无需牺牲动物，允许在所有器官的多个时间点同时收集定量数据。然而，成像以前不允许在同一动物中同时成像突变型和野生型分枝杆菌菌株。我们通过使用萤火虫（Photinus pyralis）和点击甲虫（Pyrophorus plagiophthalamus）红色荧光素酶来解决这个问题，它们发出不同的生物发光光谱，允许在感染期间同时成像两种不同的分枝杆菌菌株。我们还证明，这些生物发光报告可以用于实时评估治疗效果，极大地促进了我们在开发新型抗生素时筛选的能力。由于分枝杆菌的生长速度缓慢，迫切需要新的成像技术，因为它们可以影响新疗法的开发速度，并提高我们对毒力机制的理解和对新型候选疫苗的评估。
Molecular analysis of infectious processes in bacteria normally involves construction of isogenic mutants that can then be compared to wild type in an animal model. Pathogenesis and antimicrobial studies are complicated by variability between animals and the need to sacrifice individual animals at specific time points. Live animal imaging allows real-time analysis of infections without the need to sacrifice animals, allowing quantitative data to be collected at multiple time points in all organs simultaneously. However, imaging has not previously allowed simultaneous imaging of both mutant and wild type strains of mycobacteria in the same animal. We address this problem by using both firefly (Photinus pyralis) and click beetle (Pyrophorus plagiophthalamus) red luciferases, which emit distinct bioluminescent spectra, allowing simultaneous imaging of two different mycobacterial strains during infection. We also demonstrate that these same bioluminescence reporters can be used to evaluate therapeutic efficacy in real-time, greatly facilitating our ability to screen novel antibiotics as they are developed. Due to the slow growth rate of mycobacteria, novel imaging technologies are a pressing need, since they can they can impact the rate of development of new therapeutics as well as improving our understanding of virulence mechanisms and the evaluation of novel vaccine candidates.
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DOI：
10.1016/j.tube.2011.10.013
发表时间：
2011-12
期刊：
Tuberculosis (Edinburgh, Scotland)
影响因子：
--
作者：
Janagama HK;Hassounah HA;Cirillo SL;Cirillo JD
通讯作者：
Cirillo JD
影响因子：
11.3
作者：
Andreu N;Zelmer A;Wiles S
通讯作者：
Wiles S
影响因子：
3.6
作者：
Contag, CH;Contag, PR;Benaron, DA
通讯作者：
Benaron, DA
影响因子：
9.4
作者：
ANDREW, PW;ROBERTS, IS
通讯作者：
ROBERTS, IS
影响因子：
4.9
作者：
Hickey, MJ;Arain, TM;Stover, CK
通讯作者：
Stover, CK