Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury.
Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury.
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DOI：
10.1167/iovs.62.9.5
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发表时间：
2021-07-01
影响因子：
4.4
通讯作者：
Chauhan BC
中科院分区：
文献类型：
--
作者：
Henderson DCM;Vianna JR;Gobran J;Di Pierdomenico J;Hooper ML;Farrell SRM;Chauhan BC
描述急性（视神经横断，ONT）和慢性（实验性青光眼，EG）视神经损伤后视网膜神经节细胞（RGC）的体内树突状变化。在Thy 1-YFP小鼠中进行ONT和EG（微珠模型），其中在ONT组中在两周内以及在两个（组1和2）EG组中分别在两个月和六个月内用共焦荧光扫描激光检眼镜对整个RGC树突状乔木进行成像。Sholl分析用于量化树突结构，参数为：曲线下面积（AUC）、树突场半径、交叉峰数（PI）和与PI的距离（PD）。ONT后三天观察到树枝状变化，两周时所有参数均显着下降。在第1组EG小鼠中，EG和未处理对侧眼的平均（SD）眼内压（IOP）分别为15.2（1.1）和9.8（0.3）mmHg，AUC、PI和PD显著相应降低，但半径未降低。在第2组小鼠中，相应的IOP为13.1（1.0）和8.8（0.1）mmHg，在趋向基线前两个月达到峰值。在前两个月，AUC、PI和PD显著下降，随后无进一步变化。ONT后参数的变化率比EG快5 - 10倍。ONT后出现快速的树突状细胞变化，而EG的变化较慢，并与眼压升高水平相关。最早的改变是内神经突的丢失，而树突区没有变化。
To characterize in vivo dendritic changes in retinal ganglion cells (RGCs) after acute (optic nerve transection, ONT) and chronic (experimental glaucoma, EG) optic nerve injury. ONT and EG (microbead model) were carried out in Thy1-YFP mice in which the entire RGC dendritic arbor was imaged with confocal fluorescence scanning laser ophthalmoscopy over two weeks in the ONT group and over two and six months, respectively, in two (groups 1 and 2) EG groups. Sholl analysis was used to quantify dendritic structure with the parameters: area under the curve (AUC), radius of the dendritic field, peak number of intersections (PI), and distance to the PI (PD). Dendritic changes were observed after three days post-ONT with significant decreases in all parameters at two weeks. In group 1 EG mice, mean (SD) intraocular pressure (IOP) was 15.2 (1.1) and 9.8 (0.3) mmHg in the EG and untreated contralateral eyes, respectively, with a significant corresponding decrease in AUC, PI, and PD, but not radius. In group 2 mice, the respective IOP was 13.1 (1.0) and 8.8 (0.1) mmHg, peaking at two months before trending towards baseline. Over the first two months, AUC, PI, and PD decreased significantly, with no further subsequent changes. The rates of change of the parameters after ONT was 5 to 10 times faster than in EG. Rapid dendritic changes occurred after ONT, while changes in EG were slower and associated with level of IOP increase. The earliest alterations were loss of inner neurites without change in dendritic field.
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