Jasmonate-Elicited Stress Induces Metabolic Change in the Leaves of Leucaena leucocephala.
Jasmonate-Elicited Stress Induces Metabolic Change in the Leaves of Leucaena leucocephala.
复制标题
茉莉酸引起的应激诱导银合欢叶子的代谢变化
DOI：
10.3390/molecules23020188
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发表时间：
2018-01-24
期刊：
影响因子：
--
通讯作者：
Tsim KWK
中科院分区：
文献类型：
--
作者：
Xu Y;Tao Z;Jin Y;Chen S;Zhou Z;Gong AGW;Yuan Y;Dong TTX;Tsim KWK
将银合欢植物暴露于四种茉莉酸诱导子，即茉莉酸（JA）、甲基茉莉酸（MeJA）、茉莉酰-l-异亮氨酸（JA-Ile）和6-乙基茚满酰甘氨酸缀合物（2-[（6-乙基-1-氧代-茚满-4-羰基）-氨基]-乙酸甲酯酯）（CGM）。治疗方法是模仿食草动物和伤害压力。通过使用核磁共振波谱以及化学计量分析，包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA），确定了在四种诱导子诱导的胁迫下白头草叶子中代谢物的变化。 JA-Ile 的挑战导致乳酸 (6)、β-葡萄糖 (10)、丙氨酸 (12)、苏氨酸 (13)、类固醇 (18)、3,4-二羟基吡啶 (19) 和未鉴定化合物 20 的积累。PCA 和 PLS-DA 模型的化学计量分析表明，JA、MeJA 和 CGM 治疗触发的代谢物交替是非常小的。相比之下，JA-Ile 处理可引起叶片最显着的代谢变化。此外，在响应茉莉酸诱导的应激时检测到的新代谢物非常少。结果表明，应用诱导子后，一些代谢物浓度发生了变化，这可能与白头乳杆菌对胁迫条件的高耐受性以及较强的生态适宜性有关。
The plant Leucaena leucocephala was exposed to four jasmonate elicitors, i.e., jasmonic acid (JA), methyl jasmonic acid (MeJA), jasmonoyl-l-isoleucine (JA-Ile) and 6-ethyl indanoyl glycine conjugate (2-[(6-ethyl-1-oxo-indane-4-carbonyl)-amino]-acetic acid methyl ester) (CGM). The treatment was to mimic the herbivores and wounding stresses. By using NMR spectroscopy along with chemometric analysis, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), the changes of metabolites in the leaves of L. leucocephala were determined under the stress as induced by the four elicitors. The challenge of JA-Ile caused an accumulation of lactic acid (6), β-glucose (10), alanine (12), threonine (13), steroids (18), 3,4-dihydroxypyridine (19) and an unidentified compound 20. The chemometric analysis of the PCA and PLS-DA models indicated that the alternation of metabolites triggered by JA, MeJA, and CGM treatments were very minimum. In contrast, the treatment by JA-Ile could induce the most significant metabolic changes in the leaves. Moreover, there was very minimal new metabolite being detected in responding to the jasmonate-induced stresses. The results showed some metabolite concentrations changed after application of the elicitors, which may be related to a high level of tolerance to stress conditions as well as the strong ecological suitability of L. leucocephala.
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影响因子：
3.6
作者：
Kohl, Stefanie M.;Klein, Matthias S.;Hochrein, Jochen;Oefner, Peter J.;Spang, Rainer;Gronwald, Wolfram
通讯作者：
Gronwald, Wolfram
影响因子：
3.5
作者：
Schüler, G;Mithöfer, A;Boland, W
通讯作者：
Boland, W
影响因子：
14.9
作者：
Ulrich EL;Akutsu H;Doreleijers JF;Harano Y;Ioannidis YE;Lin J;Livny M;Mading S;Maziuk D;Miller Z;Nakatani E;Schulte CF;Tolmie DE;Kent Wenger R;Yao H;Markley JL
通讯作者：
Markley JL
影响因子：
7.3
作者：
Marti, Guillaume;Erb, Matthias;Wolfender, Jean-Luc
通讯作者：
Wolfender, Jean-Luc
影响因子：
6.1
作者：
Kim, HJ;Chen, F;Rajapakse, NC
通讯作者：
Rajapakse, NC