Characterisation of silent and active genes for a variable large protein of Borrelia recurrentis.
Characterisation of silent and active genes for a variable large protein of Borrelia recurrentis.
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DOI：
10.1186/1471-2334-2-25
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发表时间：
2002-10-14
影响因子：
3.7
通讯作者：
Kwiatkowski D
中科院分区：
文献类型：
--
作者：
Vidal V;Cutler S;Scragg IG;Wright DJ;Kwiatkowski D
我们报告的特点的可变大蛋白（vlp）基因表达的临床分离株A1的回归疏螺旋体;剂的危及生命的疾病虱传回归热。对该分离物的主要vlp蛋白进行了表征，并创建了DNA探针。将其与标准分子方法一起用于确定vlp 1B的位置。recurrentis A1基因在这两个和其他菌株。发现该分离株携带vlp 1B的沉默和表达拷贝。结果表明，在54 kbp和24 kbp的质粒上分别存在有recurrentis A1基因，而另一个不同的分离物A17仅具有沉默的vlp 1B。recurrentis A17上的54 kbp质粒。沉默和表达的vlp 1具有相同的成熟蛋白编码区，但具有不同的5'区，两者都含有不同的潜在脂蛋白前导序列。在B的A1分离物中仅转录一种形式的vlp 1。然而，该VLP 1基因的两个5'上游序列都具有细菌启动子的特征。综上所述，这些结果表明B.复发可能是由于脂蛋白前导序列和成熟蛋白编码区之间连接处可变的大蛋白和小蛋白基因的重组。然而，这一假设模型需要通过进一步鉴定其他B中表达和沉默的变体蛋白基因来验证。复发性分离物。
We report the characterisation of the variable large protein (vlp) gene expressed by clinical isolate A1 of Borrelia recurrentis; the agent of the life-threatening disease louse-borne relapsing fever. The major vlp protein of this isolate was characterised and a DNA probe created. Use of this together with standard molecular methods was used to determine the location of the vlp1B. recurrentis A1 gene in both this and other isolates. This isolate was found to carry silent and expressed copies of the vlp1B. recurrentis A1 gene on plasmids of 54 kbp and 24 kbp respectively, whereas a different isolate, A17, had only the silent vlp1B. recurrentis A17 on a 54 kbp plasmid. Silent and expressed vlp1 have identical mature protein coding regions but have different 5' regions, both containing different potential lipoprotein leader sequences. Only one form of vlp1 is transcribed in the A1 isolate of B. recurrentis, yet both 5' upstream sequences of this vlp1 gene possess features of bacterial promoters. Taken together these results suggest that antigenic variation in B. recurrentis may result from recombination of variable large and small protein genes at the junction between lipoprotein leader sequence and mature protein coding region. However, this hypothetical model needs to be validated by further identification of expressed and silent variant protein genes in other B. recurrentis isolates.
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影响因子：
64.8
作者：
PLASTERK, RHA;SIMON, MI;BARBOUR, AG
通讯作者：
BARBOUR, AG
影响因子：
4.8
作者：
Scragg, IG;Kwiatkowski, D;Morris, H
通讯作者：
Morris, H
影响因子：
82.9
作者：
Vidal, V;Scragg, IG;Kwiatkowski, D
通讯作者：
Kwiatkowski, D
DOI：
10.1093/protein/2.1.15
发表时间：
1988-04-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
KLEIN, P;SOMORJAI, RL;LAU, PCK
通讯作者：
LAU, PCK
影响因子：
15.3
作者：
NEGUSSIE, Y;REMICK, DG;GRIFFIN, GE
通讯作者：
GRIFFIN, GE