Nanocharacterization of soft biological samples in shear mode with quartz tuning fork probes.

Nanocharacterization of soft biological samples in shear mode with quartz tuning fork probes.
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DOI:
10.3390/s120404803
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发表时间:
2012
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Puig-Vidal M
Puig-Vidal M
中科院分区:
其他
文献类型:
--
作者:
Otero J;Gonzalez L;Puig-Vidal M

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石英音叉是非常好的谐振器,它们在扫描探针显微镜中的应用越来越多。然而,只有少数研究软生物样品已报告使用这些探针。在这项工作中,我们提出了开发和使用这些纳米传感器来正确处理生物样品的方法。介绍了该传感器的工作原理、制作方法和实验装置。通过使用不同的工作模式:振幅调制和不使用锁相环(PLL)和频率调制的不同样品在不同的环境中的纳米表征的结果。使用振幅调制在氮气中对铜绿假单胞菌进行成像。微接触打印的抗体在缓冲液中使用PLL的振幅调制成像。最后,使用频率调制在空气中对转移细胞成像。
Quartz tuning forks are extremely good resonators and their use is growing in scanning probe microscopy. Nevertheless, only a few studies on soft biological samples have been reported using these probes. In this work, we present the methodology to develop and use these nanosensors to properly work with biological samples. The working principles, fabrication and experimental setup are presented. The results in the nanocharacterization of different samples in different ambients are presented by using different working modes: amplitude modulation with and without the use of a Phase-Locked Loop (PLL) and frequency modulation. Pseudomonas aeruginosa bacteria are imaged in nitrogen using amplitude modulation. Microcontact printed antibodies are imaged in buffer using amplitude modulation with a PLL. Finally, metastatic cells are imaged in air using frequency modulation.
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