Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.
Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.
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活性氧催化的紫外辐射依赖性血红蛋白荧光增强
DOI:
10.1371/journal.pone.0044142
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Xu J
中科院分区:
文献类型:
--
作者:
Pan L;Wang X;Yang S;Wu X;Lee I;Zhang X;Rupp RA;Xu J
Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in fluorescence enhancement of BHb in a dose-dependant manner. Furthermore, UVI-induced fluorescence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H2O2), a type of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced fluorescence enhancement. In contrast, green light irradiation does not lead to fluorescence enhancement of BHb no matter whether H2O2 is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced fluorescence enhancement of BHb.
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影响因子:
4
作者:
Kaestner, L;Tabellion, W;Lipp, P
通讯作者:
Lipp, P
DOI:
10.1016/j.pbiomolbio.2006.02.011
发表时间:
2006-09-01
影响因子:
3.8
作者:
Gallagher, Richard P.;Lee, Tim K.
通讯作者:
Lee, Tim K.
影响因子:
1.9
作者:
Krukoski, Daniel Witchmichen;Comar, Samuel Ricardo;do Nascimento, Aguinaldo Jose
通讯作者:
do Nascimento, Aguinaldo Jose
影响因子:
2.7
作者:
Pamphilon, DH
通讯作者:
Pamphilon, DH
影响因子:
13.8
作者:
Lin, JY;Selim, MA;Pinnell, SR
通讯作者:
Pinnell, SR