Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.

Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.
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活性氧催化的紫外辐射依赖性血红蛋白荧光增强

DOI:
10.1371/journal.pone.0044142
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Xu J
Xu J
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Pan L;Wang X;Yang S;Wu X;Lee I;Zhang X;Rupp RA;Xu J

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紫外线(UV)光对生物有机体有很强的影响。血红蛋白是一种氧气转运蛋白,在维持所有脊椎动物的生命中起着不可替代的作用。在这项研究中,我们仔细研究了紫外线照射(UVI)和可见光照射对体外牛血红蛋白(BHb)荧光特性的影响。数据表明,UVI导致BHb的荧光增强呈剂量依赖性。此外,当用过氧化氢(H2O2)(一种活性氧(ROS))预处理BHb时,紫外线诱导的荧光增强显著增强。同时,水溶性抗氧化剂维生素C抑制这种紫外线诱导的荧光增强。而无论H2O2是否作用于BHb溶液,绿光照射均不会导致BHb的荧光增强。综上所述,这些结果表明活性氧的催化作用和紫外线依赖性照射在紫外线诱导BHb荧光增强的过程中起着两个关键作用。
Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in fluorescence enhancement of BHb in a dose-dependant manner. Furthermore, UVI-induced fluorescence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H2O2), a type of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced fluorescence enhancement. In contrast, green light irradiation does not lead to fluorescence enhancement of BHb no matter whether H2O2 is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced fluorescence enhancement of BHb.
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