Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique.
Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique.
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DOI：
10.3390/ijms13010260
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发表时间：
2012
影响因子：
5.6
通讯作者：
Chen BH
中科院分区：
文献类型：
--
作者：
Chen HJ;Inbaraj BS;Chen BH
建立了中草药台湾蒲公英中酚酸类和黄酮类化合物的液相色谱-串联质谱（LC-MS/MS）分析方法。首先，在60 °C水浴中用50%乙醇提取酚酸和黄酮类化合物3 h，并最终通过使用C18柱分离成酸性部分和中性部分。采用Gemini C18色谱柱，0.1%甲酸-乙腈梯度洗脱，流速1.0mL/min，在68 min内分离了29种化合物，根据保留行为、吸收光谱和质谱，鉴定了19种酚酸类化合物和10种黄酮类化合物。台湾产的含量范围为14.1 ~ 10，870.4 μg/g，台湾产的含量范围为9.9 ~ 325.8 μg/g。为了进一步鉴定黄酮类化合物，采用柱后衍生法，包括位移试剂，如乙酸钠或氯化铝和吸收光谱特性进行了比较，没有或有位移试剂。采用内标法定量测定了黄酮类化合物的含量，而（±）柚皮素则适用于黄酮类化合物的定量测定。所开发的LC-MS/MS方法显示出高重现性，如从酚酸的日内和日间变异性的相对标准偏差（RSD）值分别为1.0-6.8%和2.0-7.7%以及黄酮类化合物的3.7-7.4%和1.5-8.1%所证明的，可用于中草药和保健品中酚酸类和黄酮类化合物的同时测定。
A liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed for the determination of phenolic acids and flavonoids in a medicinal Chinese herb Taraxacum formosanum Kitam. Initially, both phenolic acids and flavonoids were extracted with 50% ethanol in a water-bath at 60 °C for 3 h and eventually separated into acidic fraction and neutral fraction by using a C18 cartridge. A total of 29 compounds were separated within 68 min by employing a Gemini C18 column and a gradient solvent system of 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. Based on the retention behavior as well as absorption and mass spectra, 19 phenolic acids and 10 flavonoids were identified and quantified in T. formosanum, with the former ranging from 14.1 μg/g to 10,870.4 μg/g, and the latter from 9.9 μg/g to 325.8 μg/g. For further identification of flavonoids, a post-column derivatization method involving shift reagents such as sodium acetate or aluminum chloride was used and the absorption spectral characteristics without or with shift reagents were compared. An internal standard syringic acid was used for quantitation of phenolic acids, whereas (±) naringenin was found suitable for quantitation of flavonoids. The developed LC-MS/MS method showed high reproducibility, as evident from the relative standard deviation (RSD) values for intra-day and inter-day variability being 1.0–6.8% and 2.0–7.7% for phenolic acids and 3.7–7.4% and 1.5–8.1% for flavonoids, respectively, and thus may be applied for simultaneous determination of phenolic acids and flavonoids in Chinese herb and nutraceuticals.
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影响因子：
8.8
作者：
Elizabeth Rocha-Guzman, Nuria;Alberto Gallegos-Infante, Jose;Azalia Lujan-Garcia, Blanca
通讯作者：
Azalia Lujan-Garcia, Blanca
DOI：
10.1016/j.jpba.2009.09.006
发表时间：
2010-02-05
影响因子：
3.4
作者：
Inbaraj, B. Stephen;Lu, H.;Chen, B. H.
通讯作者：
Chen, B. H.
影响因子：
6.1
作者：
Mancek, B;Kreft, S
通讯作者：
Kreft, S
影响因子：
6.1
作者：
Koo, HN;Hong, SH;Kim, HM
通讯作者：
Kim, HM
影响因子：
4.3
作者：
Hu, C;Kitts, DD
通讯作者：
Kitts, DD