The effects of decorin and HGF-primed vocal fold fibroblasts in vitro and ex vivo in a porcine model of vocal fold scarring.
The effects of decorin and HGF-primed vocal fold fibroblasts in vitro and ex vivo in a porcine model of vocal fold scarring.
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DOI：
10.1002/lary.21087
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发表时间：
2010-11
期刊：
影响因子：
2.6
通讯作者：
Wells, Alan
中科院分区：
文献类型：
--
作者：
Krishna, Priya;Regner, Michael;Palko, Joel;Liu, Fang;Abramowitch, Steve;Jiang, Jack;Wells, Alan
声带损伤可能是不可逆的，导致声带瘢痕，具有永久性的功能影响，没有最佳的治疗方法。采用细胞迁移法和免疫印迹法，以及对猪喉部和切除的猪声带的功能测量，在猪声带瘢痕模型中测试了decorin和引物声带成纤维细胞的体外作用。体外：对原代猪声带成纤维细胞（pvff）进行细胞迁移实验（划痕），并以装饰素20 μg/mL、肝细胞生长因子（HGF） 200 ng/mL、表皮生长因子（EGF） 1 nM或转化生长因子-β1 10 ng/mL处理。细胞也进行了decorin剂量反应测试。在MetaMorph®Imaging中分析划痕试验；细胞裂解液中MMP-8和I型胶原的含量。11头猪接受单侧声带剥离手术。术后第3天，受试者在患声带浅表注射decorin 20 μg/mL或1 × 106 hgf - primal成纤维细胞。切除喉部，用于离体喉部检测或流变学检测。划痕实验显示，用decorin或HGF处理的pvff细胞迁移明显减少。随着decorin剂量的增加，细胞中MMP-8的生成增加（P <0.01），胶原蛋白减少。粘弹性测量表明，decorin处理的样品的刚度有所增加。离体空气动力学测试表明，decorin治疗喉部的发声效率评分有所提高。Decorin在体外对PVFF迁移有明显的影响，似乎增加了声带硬度，但要么不改变，要么稍微增加了声带效率。证据等级：5
Vocal fold injury can be irreversible, leading to vocal fold scarring, with permanent functional effects and no optimal treatment. A porcine model of vocal fold scarring was used to test effects of decorin and primed vocal fold fibroblasts in vitro using a cell migration assay and immunoblotting, and by using functional measurements of porcine larynges and excised porcine vocal folds. In vitro: primary pig vocal fold fibroblasts (PVFFs) were subjected to cell migration assays (scratch) and treated with decorin 20 μg/mL, hepatocyte growth factor (HGF) 200 ng/mL, epidermal growth factor (EGF) 1 nM, or transforming growth factor-β1 10 ng/mL. Cells also underwent decorin dose response testing. Scratch assays were analyzed in MetaMorph® Imaging; cell lysates were processed for MMP-8 and type I collagen content. Eleven pigs underwent unilateral vocal fold stripping procedures. At day 3 postoperatively, subjects underwent superficial injection into the affected vocal fold either with decorin 20 μg/mL or 1 × 106 HGF-primed fibroblasts. Larynges were harvested and either used for ex vivo laryngeal testing or for rheological testing. Scratch assay indicated significantly reduced cell migration in PVFFs treated with decorin or HGF. MMP-8 production was increased (P <0.01) and collagen was decreased in cells treated with decorin at increasing doses. Viscoelastic measurements suggested somewhat increased stiffness for decorin treated samples. Ex vivo aerodynamic testing suggested improved vocal efficiency scores in decorin-treated larynges. Decorin has a noticeable effect on PVFF migration in vitro and appears to increase vocal fold stiffness but either does not change or slightly increases vocal efficiency. Level of evidence: 5
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影响因子：
2.4
作者：
Chan, RW;Titze, IR
通讯作者：
Titze, IR
影响因子：
6.2
作者：
Douglas, Timothy;Heinemann, Sascha;Worch, Hartmut
通讯作者：
Worch, Hartmut
影响因子：
--
作者：
Xu, Chet C.;Chan, Roger W.;Tirunagari, Neeraj
通讯作者：
Tirunagari, Neeraj
影响因子：
3.4
作者：
Krishna, Priya;Rosen, Clark A.;Hebda, Patricia A.
通讯作者：
Hebda, Patricia A.
DOI：
10.1097/01.moo.0000162261.49739.b7
发表时间：
2005-06-01
影响因子：
1.6
作者：
Hirano, Shigeru
通讯作者：
Hirano, Shigeru