Effects of selective medium on lipid accumulation of chlorellas and screening of high lipid mutants through ultraviolet mutagenesis
Effects of selective medium on lipid accumulation of chlorellas and screening of high lipid mutants through ultraviolet mutagenesis
复制标题
选择培养基对小球藻脂质积累的影响及紫外诱变筛选高脂突变体
DOI：
10.5897/ajar10.810
复制
发表时间：
2011-08
影响因子：
--
通讯作者：
Fei, Xiaowen
中科院分区：
文献类型：
--
作者：
Deng, Xiaodong;Li, Yajun;Fei, Xiaowen
从海南热带淡水中分离到两种微藻Y019和Y041。通过18S rDNA序列的系统发育分析，鉴定为普通小球藻（Chlorella vulgaris）和核核小球藻（Chlorella pyrenoidosa）。随后，在25℃、230 r/min、24 h全光[z1]、50-110 μE/m2/s条件下，分别与C. vulgaris FACHB-31在SE、BG11、HSM1、DS四种选择性培养基中培养。检测这些培养基对细胞生长和脂质含量的影响。结果表明，HSM1培养基更适合小球藻生长，而DS培养基更有利于脂质积累。在DS培养基中，12 d时，C. vulgaris Y019的脂质含量是其他品种的1.84 ~ 2.64倍。因此，本地分离藻类C. vulgaris Y019被认为是生产生物柴油的理想原料。在此基础上，我们对C. vulgaris Y019进行了紫外诱变。在HSM和HSM- n培养基上共筛选了1086株藻类产脂。结果表明：在HSM培养基中，突变体Y019-M57和Y019-M66为高脂质突变体，而在HSM- n培养基中，突变体Y019-M67为高脂质突变体。这些突变体为进一步的研究和商业应用奠定了基础。
Two micro-algae Y019 and Y041 were isolated from tropical fresh water in Hainan, China. Throughphylogenetic analysis of 18S rDNA sequences, they had been identified as Chlorella vulgaris and Chlorella pyrenoidosa. Subsequently, both of them and C. vulgaris FACHB-31 were cultured respectively in four selective medium (SE, BG11, HSM1, DS) at 25°C, 230 r/min, 24 h full light[z1], 50-110 μE/m2/s. Effects of these media on the cell growth and lipid content were detected. The results showed that, the HSM1 medium is more suitable for the growth of Chlorella, while the DS medium is more conducive to the lipid accumulation. In the DS medium, the lipid content of C. vulgaris Y019 was 1.84 ~ 2.64-fold than others at 12 days. So the local separated algae C. vulgaris Y019 is thought of an ideal raw material for biodiesel production. After that, we conducted Ultraviolet mutagenesis for C. vulgaris Y019. A total of 1086 algae strains had been screened for lipid production in HSM and HSM-N media.. Results showed: in HSM medium, mutants Y019-M57 and Y019-M66 were identified as high lipid producing mutants, while in HSM-N medium Y019-M67 was detected as high lipid content mutants. These mutants laid the foundation for further research and commercial application.
Key words: Chlorella, culture medium, Ultraviolet mutants, lipid accumulation.
登录
查看更多内容
微藻：一种有前途的生物柴油原料
影响因子：
--
作者：
Xiaodong Deng;Yajun Li;Xiaowen Fei
通讯作者：
Xiaowen Fei
影响因子：
11.4
作者：
Feng, Yujie;Li, Chao;Zhang, Dawei
通讯作者：
Zhang, Dawei
影响因子：
2.2
作者：
Chen, Wei;Zhang, Chengwu;Hu, Qiang
通讯作者：
Hu, Qiang
影响因子：
--
作者：
Deng, Xiaodong;Eriksson, Mats
通讯作者：
Eriksson, Mats
影响因子：
2.2
作者：
Gao, Chunfang;Xiong, Wei;Wu, Qingyu
通讯作者：
Wu, Qingyu