Plastic-based lateral flow immunoassay device for electrochemical detection of NT-proBNP.
Plastic-based lateral flow immunoassay device for electrochemical detection of NT-proBNP.
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DOI：
10.1039/d2an00685e
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发表时间：
2022-05-30
期刊：
影响因子：
4.2
通讯作者：
Crooks, Richard M.
中科院分区：
文献类型：
--
作者：
Raj, Nikhil;Crooks, Richard M.
在这里，我们报告了一种易于制造的、基于塑料的侧向流动装置，用于进行金属免疫分析。这种设备被称为ocFlow，以强调明渠设计。我们已经证明，ocFlow能够用于基于磁性微珠(MµB)的金属免疫分析，用于检测两种类型的免疫结合物：模型复合材料(MC)和心力衰竭标志物NT-proBNP的夹心免疫分析。在这两种分析中，银纳米颗粒(AgNPs)被用作电化学可检测的标记。使用OCFLOW装置可以检测到NT-ProBNP和MC的浓度，分别低至750.0 pm和10.0 pm。从本文给出的结果可以得出四个关键结论。首先，附着在MµB上的免疫结合物可以通过流体动力和毛细管压力被动泵相结合的方式在流动通道中输送。其次，ocFlow器件能够在芯片上存储、分解和形成MC和NT-proBNP复合材料。第三，通过用洗涤缓冲液冲洗电极，可以对血清中悬浮的分析物进行电化学检测。最后，也可能是最重要的是，当必要的试剂以干燥形式储存在设备上时，该分析是定量的，并且对NT-proBNP具有高皮摩尔范围的检测下限。研制了一种基于塑料的低成本侧向流动装置，利用电化学金属免疫分析技术检测心力衰竭标志物NT-proBNP。
Here we report an easily fabricated, plastic-based lateral flow device for carrying out metalloimmunoassays. The device is called ocFlow to emphasize the open-channel design. We have shown that the ocFlow is capable of magnetic microbead (MµB)-based metalloimmunoassays for the detection of two types of immunoconjugates: a model composite (MC) and a sandwich immunoassay for the heart failure marker NT-proBNP. In both assays, Ag nanoparticles (AgNPs) were used as electrochemically detectable labels. NT-proBNP and MC concentrations as low as 750.0 pM and 10.0 pM, respectively, could be detected using the ocFlow device. Four key conclusions can be drawn from the results presented herein. First, immunoconjugates attached to the MµBs can be transported in the flow channel using combined hydrodynamic and capillary pressure passive pumping. Second, the ocFlow device is capable of on-chip storage, resolvation, and conjugate formation of both the MC and NT-proBNP composites. Third, electrochemical detection can be conducted on analytes suspended in serum by rinsing the electrodes with a wash buffer. Finally, and perhaps most significantly, the assay is quantitative and has a detection limit for NT-proBNP in the high picomolar range when the necessary reagents are stored on the device in a dry form. A low-cost plastic-based lateral flow device was developed to detect NT-proBNP, a heart failure marker, using the electrochemical metalloimmunoassay technique.
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影响因子：
8.9
作者：
Pollok NE;Peng Y;Rabin C;Richards I;Crooks RM
通讯作者：
Crooks RM
影响因子：
6.4
作者：
Koczula KM;Gallotta A
通讯作者：
Gallotta A
DOI：
10.1073/pnas.1302566110
发表时间：
2013-06-18
影响因子：
11.1
作者：
Casavant, Benjamin P.;Berthier, Erwin;Beebe, David J.
通讯作者：
Beebe, David J.
利用石墨烯场效应晶体管生物传感器检测全血中心力衰竭相关生物标志物
影响因子：
12.6
作者：
Lei, Yong-Min;Xiao, Meng-Meng;Zhang, Guo-Jun
通讯作者：
Zhang, Guo-Jun
影响因子：
8.9
作者：
Cunningham, Josephine C.;Kogan, Molly R.;Crooks, Richard M.
通讯作者：
Crooks, Richard M.