Lithography-free fabrication of reconfigurable substrate topography for contact guidance.
Lithography-free fabrication of reconfigurable substrate topography for contact guidance.
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DOI：
10.1016/j.biomaterials.2014.10.078
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发表时间：
2015-01
期刊：
影响因子：
14
通讯作者：
Bettinger CJ
中科院分区：
文献类型：
--
作者：
Pholpabu P;Kustra S;Wu H;Balasubramanian A;Bettinger CJ
哺乳动物细胞在体内和体外都能检测和响应天然和合成生物材料中出现的地形线索。微纳米结构影响许多表型的黏附、形态、增殖、迁移和分化。尽管支持细胞-地形相互作用的机制仍然难以捉摸，但具有明确微结构和纳米结构的合成底物是阐明这些反应的起源的重要工具。具有可重构形的衬底是可取的，因为可编程提示可以与动态细胞响应相协调。在这里，我们提出了一种无光刻的制造技术，它可以使用一种最大限度地减少所施加刺激的混杂效应的驱动机制来可逆地呈现地形线索。这种方法利用了双层衬底材料中应变诱导的屈曲不稳定性，双层衬底材料具有刚性的均匀氧化硅薄膜，这些氧化硅薄膜热沉积在弹性衬底上。所得到的表面能够在三种不同的状态之间进行可逆：平面基片(A=1.53±0.55 nm，均方根=0.317±0.048 nm)，平行波纹光栅阵列(A||=483.6±7.8 nm，λ|=4.78±0.16μm)，垂直波纹光栅阵列(A⊥=429.3±5.8 nm，λ⊥=4.95±0.36μm)。用光学显微镜测量了3T3成纤维细胞对这些表面的细胞骨架动态变化。在从平面特征转换为地形特征的动态基质(Flat-WAVY)上培养的成纤维细胞大体形态发生了强劲而迅速的变化，5分钟后圆形从0.30±0.13减少到0.15±0.08。相反，Flat-WAVY-Flat的动态衬底序列不会显著改变总体稳态形态。综上所述，呈现可逆拓扑结构的底物可以阐明细胞-拓扑相互作用的动态方面。
Mammalian cells detect and respond to topographical cues presented in natural and synthetic biomaterials both in vivo and in vitro. Micro- and nano-structures influence the adhesion, morphology, proliferation, migration, and differentiation of many phenotypes. Although the mechanisms that underpin cell-topography interactions remain elusive, synthetic substrates with well-defined micro- and nano-structures are important tools to elucidate the origin of these responses. Substrates with reconfigurable topography are desirable because programmable cues can be harmonized with dynamic cellular responses. Here we present a lithography-free fabrication technique that can reversibly present topographical cues using an actuation mechanism that minimizes the confounding effects of applied stimuli. This method utilizes strain-induced buckling instabilities in bi-layer substrate materials with rigid uniform silicon oxide membranes that are thermally deposited on elastomeric substrates. The resulting surfaces are capable of reversible of substrates between three distinct states: flat substrates (A = 1.53 ± 0.55 nm, Rms = 0.317 ± 0.048 nm); parallel wavy grating arrays (A|| = 483.6 ± 7.8 nm and λ|| = 4.78 ± 0.16 μm); perpendicular wavy grating arrays (A⊥ = 429.3 ± 5.8 nm; λ⊥ = 4.95 ± 0.36 μm). The cytoskeleton dynamics of 3T3 fibroblasts in response to these surfaces was measured using optical microscopy. Fibroblasts cultured on dynamic substrates that are switched from flat to topographic features (FLAT-WAVY) exhibit a robust and rapid change in gross morphology as measured by a reduction in circularity from 0.30 ± 0.13 to 0.15 ± 0.08 after 5 min. Conversely, dynamic substrate sequences of FLAT-WAVY-FLAT do not significantly alter the gross steady-state morphology. Taken together, substrates that present topographic structures reversibly can elucidate dynamic aspects of cell-topography interactions.
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影响因子：
2.4
作者：
Cheng, Chao-Min;Steward, Robert L., Jr.;LeDuc, Philip R.
通讯作者：
LeDuc, Philip R.
影响因子：
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作者：
Hakkinen, Kirsi M.;Harunaga, Jill S.;Yamada, Kenneth M.
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作者：
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通讯作者：
Curtis, ASG
影响因子：
14
作者：
Idota, Naokazu;Tsukahara, Takahiko;Kitamori, Takehiko
通讯作者：
Kitamori, Takehiko