Reconstituting ring-rafts in bud-mimicking topography of model membranes.
Reconstituting ring-rafts in bud-mimicking topography of model membranes.
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DOI：
10.1038/ncomms5507
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发表时间：
2014-07-24
影响因子：
16.6
通讯作者：
Lee, Sin-Doo
中科院分区：
文献类型：
--
作者：
Ryu, Yong-Sang;Lee, In-Ho;Suh, Jeng-Hun;Park, Seung Chul;Oh, Soojung;Jordan, Luke R.;Wittenberg, Nathan J.;Oh, Sang-Hyun;Jeon, Noo Li;Lee, Byoungho;Parikh, Atul N.;Lee, Sin-Doo
在囊泡运输和包膜病毒的释放过程中，出芽和分裂过程以蛋白质或脂质介导的方式动态地重塑供体细胞膜。在所有情况下，除了产生或缓解的曲率应力，芽招募特定的脂质和蛋白质从供体膜通过限制性扩散的封闭拓扑结构的环状筏域的发展。在这里，通过重建的芽地形模型膜，我们证明了优先本地化的胆固醇和鞘磷脂富集的微区的衣领带的芽颈接口与供体膜。动态膜重组的重演的几何方法，从纳米到微米尺度的局部曲率半径，提供了重要的线索，了解芽地形的积极作用，在膜出芽的关键信号蛋白的分选和迁移机制。 细胞膜上的出芽过程涉及表面几何形状的扭曲，由膜组分的分选驱动。在这里，作者模拟了一个萌芽过程，并观察到在高曲率区域，特别是在芽颈周围，材料自发重新排序，产生环筏型结构。
During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol- and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding. Budding processes on cell membranes involve distortions of surface geometry, driven by the sorting of membrane components. Here, the authors model a budding process and observe spontaneous reordering of materials in the areas of high curvature, especially around bud-necks, producing ring-raft type structures.
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影响因子：
64.5
作者：
Hurley JH;Boura E;Carlson LA;Różycki B
通讯作者：
Różycki B
影响因子：
4.8
作者：
Garcia-Saez, Ana J.;Chiantia, Salvatore;Schwille, Petra
通讯作者：
Schwille, Petra
影响因子：
3.9
作者：
Chen, I-Jane;Lindner, Erno
通讯作者：
Lindner, Erno
影响因子：
3.7
作者：
Kurczy ME;Mellander LJ;Najafinobar N;Cans AS
通讯作者：
Cans AS
影响因子：
3.4
作者：
Buboltz, JT;Feigenson, GW
通讯作者：
Feigenson, GW