Potential of Tamarind Shell Extract against Oxidative Stress In Vivo and In Vitro.
Potential of Tamarind Shell Extract against Oxidative Stress In Vivo and In Vitro.
复制标题
罗望子壳提取物在体内和体外抗氧化应激的潜力。
DOI：
10.3390/molecules28041885
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发表时间：
2023-02-16
期刊：
影响因子：
4.6
通讯作者：
He, Rongrong
中科院分区：
文献类型：
--
作者：
Li, Weixi;Huang, Rongping;Han, Shaocong;Li, Xiyou;Gong, Haibiao;Zhang, Qiongyi;Yan, Changyu;Li, Yifang;He, Rongrong
关键词：
罗望子壳中含有丰富的黄酮类化合物，具有良好的生物活性。本研究旨在分析罗望子壳提取物（TSE）的化学成分，并研究TSE的体内外抗氧化能力。采用95%乙醇水溶液提取罗望子壳中的挥发性成分，采用超高效液相色谱-电喷雾串联质谱（UPLC-MS/MS）法测定其化学成分。采用氧自由基吸收能力（ORAC）法评价TSE体外清除自由基的活性。在2，2-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）刺激的ADTC 5细胞和叔丁基过氧化氢（t-BHP）暴露的斑马鱼中进一步评估TSE的抗氧化作用。结果表明，在TSE中共检测到8种黄酮类化合物，分别为（+）-儿茶素、花旗松素、杨梅素、圣草酚、木樨草素、桑色素、芹菜素和柚皮素，含量分别为5.287、8.419、4.042、6.583、3.421、4.651、0.2027和0.6234 mg/g。ORAC实验表明，TSE和黄酮类化合物具有较强的体外自由基清除活性。此外，TSE显着降低ROS和MDA水平，但恢复超氧化物歧化酶的活性在AAPH处理的ATDC 5细胞和t-BHP暴露的斑马鱼。黄酮类化合物在ATDC 5细胞和斑马鱼中也表现出良好的抗氧化活性。综上所述，本研究提示了罗望子壳及其主要黄酮类化合物在体内外的自由基清除能力和抗氧化潜力，为罗望子壳的开发利用提供了理论依据。
Tamarind shell is rich in flavonoids and exhibits good biological activities. In this study, we aimed to analyze the chemical composition of tamarind shell extract (TSE), and to investigate antioxidant capacity of TSE in vitro and in vivo. The tamarind shells were extracted with 95% ethanol refluxing extraction, and chemical constituents were determined by ultra-performance chromatography–electrospray tandem mass spectrometry (UPLC-MS/MS). The free radical scavenging activity of TSE in vitro was evaluated using the oxygen radical absorbance capacity (ORAC) method. The antioxidative effects of TSE were further assessed in 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)-stimulated ADTC5 cells and tert-butyl hydroperoxide (t-BHP)-exposed zebrafish. A total of eight flavonoids were detected in TSE, including (+)-catechin, taxifolin, myricetin, eriodictyol, luteolin, morin, apigenin, and naringenin, with the contents of 5.287, 8.419, 4.042, 6.583, 3.421, 4.651, 0.2027, and 0.6234 mg/g, respectively. The ORAC assay revealed TSE and these flavonoids had strong free radical scavenging activity in vitro. In addition, TSE significantly decreased the ROS and MDA levels but restored the SOD activity in AAPH-treated ATDC5 cells and t-BHP-exposed zebrafish. The flavonoids also showed excellent antioxidative activities against oxidative damage in ATDC5 cells and zebrafish. Overall, the study suggests the free radical scavenging capacity and antioxidant potential of TSE and its primary flavonoids in vitro and in vivo and will provide a theoretical basis for the development and utilization of tamarind shell.
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