Old and new stories: revelations from functional analysis of the bovine mammary transcriptome during the lactation cycle.
Old and new stories: revelations from functional analysis of the bovine mammary transcriptome during the lactation cycle.
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DOI：
10.1371/journal.pone.0033268
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Loor JJ
中科院分区：
文献类型：
--
作者：
Bionaz M;Periasamy K;Rodriguez-Zas SL;Everts RE;Lewin HA;Hurley WL;Loor JJ
在相对于分娩的- 30、- 15、1、15、30、60、120、240和300 d时检测奶牛乳腺转录组。在整个哺乳期共发现6382个错误发现率≤0.001的差异表达基因（DEG）。在60天和120天观察到最多的DEG (> 3500 DEG)，而在- 15天和1天以及120天和240天观察到的连续时间点之间变化最大。微阵列数据的功能分析使用动态影响方法（DIA）进行。DIA分析发现，KEGG通路受影响最大，并诱导了“半乳糖代谢”、“糖基磷脂酰肌醇（GPI）锚点生物合成”和“PPAR信号传导”；而“抗原加工和呈递”是最受抑制的。对结果的综合解释表明，哺乳期间代谢总体增加，特别是碳水化合物和脂质的合成。DIA分析还表明，氨基酸作为能量来源的利用程度显著，蛋白质输出量增加，蛋白质合成机制和细胞周期减少。通过对Gene Ontology和其他数据库的DIA分析，发现在哺乳期间高尔基体和血管生成的诱导，以及免疫细胞活性/迁移和染色体修饰的抑制。所有在泌乳过程中受到高度影响和激活的功能在泌乳开始时被明显激活，在产奶量下降时被抑制。总体分析表明，牛乳腺在很大程度上依赖于一个协调的转录调节来开始和结束泌乳。使用DIA进行的功能分析强调了与乳糖合成、脂质代谢、蛋白质合成、高尔基体、运输、细胞周期/死亡、表观遗传调控、血管生成和哺乳期间免疫功能相关的基因的重要性。
The cow mammary transcriptome was explored at −30, −15, 1, 15, 30, 60, 120, 240, and 300 d relative to parturition. A total of 6,382 differentially expressed genes (DEG) at a false discovery rate ≤0.001 were found throughout lactation. The greatest number of DEG (>3,500 DEG) was observed at 60 and 120 d vs. −30 d with the largest change between consecutive time points observed at −15 vs. 1 d and 120 vs. 240 d. Functional analysis of microarray data was performed using the Dynamic Impact Approach (DIA). The DIA analysis of KEGG pathways uncovered as the most impacted and induced ‘Galactose metabolism’, ‘Glycosylphosphatidylinositol (GPI)-anchor biosynthesis’, and ‘PPAR signaling’; whereas, ‘Antigen processing and presentation’ was among the most inhibited. The integrated interpretation of the results suggested an overall increase in metabolism during lactation, particularly synthesis of carbohydrates and lipid. A marked degree of utilization of amino acids as energy source, an increase of protein export, and a decrease of the protein synthesis machinery as well cell cycle also were suggested by the DIA analysis. The DIA analysis of Gene Ontology and other databases uncovered an induction of Golgi apparatus and angiogenesis, and the inhibition of both immune cell activity/migration and chromosome modifications during lactation. All of the highly-impacted and activated functions during lactation were evidently activated at the onset of lactation and inhibited when milk production declined. The overall analysis indicated that the bovine mammary gland relies heavily on a coordinated transcriptional regulation to begin and end lactation. The functional analysis using DIA underscored the importance of genes associated with lactose synthesis, lipid metabolism, protein synthesis, Golgi, transport, cell cycle/death, epigenetic regulation, angiogenesis, and immune function during lactation.
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影响因子：
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