Psychological stress delays periodontitis healing in rats: the involvement of basic fibroblast growth factor.
Psychological stress delays periodontitis healing in rats: the involvement of basic fibroblast growth factor.
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心理压力延迟大鼠牙周炎愈合：碱性成纤维细胞生长因子的参与
DOI：
10.1155/2012/732902
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发表时间：
2012
影响因子：
4.6
通讯作者：
Chen YJ
中科院分区：
文献类型：
--
作者：
Zhao YJ;Li Q;Cheng BX;Zhang M;Chen YJ
Objective.探讨心理应激对大鼠牙周炎愈合的影响及碱性成纤维细胞生长因子（bFGF）在牙周炎愈合过程中的作用。方法.将96只大鼠随机分为对照组、牙周炎组和牙周炎+应激组。然后，在基线和第1、2和4周处死大鼠。观察牙周炎愈合情况，免疫组化法检测牙周组织中白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）、碱性成纤维细胞生长因子（bFGF）的表达。结果应激组大鼠体重增长减少，行为学改变，血清皮质酮和ACTH水平升高（P < 0.05）。应激组炎症浸润面积、牙槽骨丢失、附着丧失、IL-1β和TNF-α表达在2、4周均高于牙周炎组（P < 0.05）。实验性牙周炎大鼠牙周组织中bFGF表达明显降低（P < 0.05），应激组bFGF表达恢复较牙周炎组慢（P < 0.05）。炎性细胞因子与bFGF呈负相关。结论心理应激可延缓大鼠牙周炎的愈合，其机制可能与下调牙周膜中bFGF的表达有关。
Objective. To evaluate the effects of psychological stress on periodontitis healing in rats and the contribution of basic fibroblast growth factor (bFGF) expression to the healing process. Methods. Ninety-six rats were randomly distributed into control group, periodontitis group, and periodontitis plus stress group. Then, the rats were sacrificed at baseline and week(s) 1, 2, and 4. The periodontitis healing condition was assessed, and the expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and bFGF were tested by immunohistochemistry. Results. The stressed rats showed reduced body weight gain, behavioral changes, and increased serum corticosterone and ACTH levels (P < 0.05). The surface of inflammatory infiltrate, alveolar bone loss, attachment loss, and expression of IL-1β and TNF-α in the stress group were higher than those in the periodontitis group at weeks 2 and 4 (P < 0.05). Rats with experimental periodontitis showed decreased bFGF expression (P < 0.05), and the recovery of bFGF expression in the stress group was slower than that in the periodontitis group (P < 0.05). Negative correlations between inflammatory cytokines and bFGF were detected. Conclusion. Psychological stress could delay periodontitis healing in rats, which may be partly mediated by downregulation of the expression of bFGF in the periodontal ligament.
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DOI：
10.1590/s0100-879x2012007500102
发表时间：
2012-10
期刊：
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
影响因子：
--
作者：
Lv X;Li Q;Wu S;Sun J;Zhang M;Chen YJ
通讯作者：
Chen YJ
影响因子：
4.1
作者：
Brechter, Anna Bernhold;Persson, Emma;Lerner, Ulf H.
通讯作者：
Lerner, Ulf H.
影响因子：
3.5
作者：
Camargo, PM;Lekovic, V;Kenney, EB
通讯作者：
Kenney, EB
影响因子：
3.8
作者：
Fentoglu, O.;Kirzioglu, F. Y.;Sert, T.
通讯作者：
Sert, T.
影响因子：
1.9
作者：
Gaspersic, R;Stiblar-Martincic, D;Skaleric, U
通讯作者：
Skaleric, U