Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors.
Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors.
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DOI：
10.1186/s13046-021-02105-3
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发表时间：
2021-09-27
期刊：
影响因子：
--
通讯作者：
Cui C
中科院分区：
文献类型：
--
作者：
Ye L;Chen Y;Mao J;Lei X;Yang Q;Cui C
在一个单一的纳米平台内开发结合诊断和治疗功能的新型纳米材料对肿瘤治疗诊断学具有挑战性。我们合成了树枝状大分子修饰的金纳米棒，用于结肠癌的联合基因治疗和光热治疗（PTT）。用GX 1肽（一种环状7肽，CGNSNPKSC）修饰聚酰胺-胺树枝状大分子（PAMAM，G3）接枝的金纳米棒。将所获得的Au NR@ PAMAM-GX 1作为基因（FAM 172 A，调节结肠癌细胞的增殖和凋亡）的基因递送载体，用于结肠癌细胞（HCT-8细胞）的光热疗法（PTT）和基因疗法的组合。此外，Au NR的CT成像功能可为结肠癌的诊断提供影像学依据。结果表明，Au NR@ PAMAM-GX 1能够以优异的转染效率特异性地将FAM 172 A递送至癌细胞。用Au NR@ PAMAM-GX 1/FAM 172 A在激光照射下处理的HCT-8细胞的存活率为20.45%，远低于其他单一模式PTT治疗或单一模式基因治疗的存活率。此外，动物实验结果证实，Au NR@ PAMAM-GX 1/FAM 172 A复合物经尾静脉注射后可实现肿瘤热成像、靶向CT成像、PTT和基因治疗。我们的研究结果表明，合成的Au NR@ PAMAM-GX 1为发挥抗肿瘤作用和提高肿瘤诊断水平提供了一个简单的平台。在线版本包含补充材料，可通过10.1186/s13046-021-02105-3获得。
The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics. We synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au NR@PAMAM-GX1 are proposed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer. The results display that Au NR@PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, targeted CT imaging, PTT and gene therapy after tail vein injection. Our findings demonstrate that the synthesized Au NR@PAMAM-GX1 offer a facile platform to exert antitumor and improve the diagnostic level of tumor. The online version contains supplementary material available at 10.1186/s13046-021-02105-3.
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