Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis.
Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis.
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DOI：
10.1371/journal.pone.0058406
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kalloniatis M
中科院分区：
文献类型：
--
作者：
Nivison-Smith L;Collin SP;Zhu Y;Ready S;Acosta ML;Hunt DM;Potter IC;Kalloniatis M
七鳃鳗是脊椎动物进化中两个幸存的无颌阶段之一，因此是阐明视觉系统进化的理想候选者。本研究研究了南半球七鳃鳗(Geotria Australis)在幼体向下游迁徙到大海以及成年和性成熟成体向其产卵区上游迁徙过程中的视网膜氨基酸神经化学。谷氨酸和牛磺酸分布在视网膜各处，而GABA和甘氨酸仅限于视网膜内部的神经元，与大多数其他脊椎动物的模式相匹配。谷氨酰胺和天冬氨酸的免疫反应与Müler细胞的形态密切相关。在不同的迁移阶段，主要神经递质谷氨酸、GABA和甘氨酸的分布几乎没有差异，但与视网膜代谢有关的氨基酸谷氨酰胺和天冬氨酸的变化明显。牛磺酸的免疫反应性在迁徙阶段之间大多是保守的，这与它在初级细胞功能中的作用一致，如渗透调节。用AgB探针进一步研究谷氨酸信号通路，发现AgB进入光感受器和水平细胞，随后聚集在视网膜内神经元。在AGB谱上的相似性证实了谷氨酸神经传递的保守性。最后，钙结合蛋白、钙结合蛋白和钙视黄素定位于视网膜内层，而恢复素定位于光感受器。总体而言，七鳃鳗视网膜中主要氨基酸神经递质和钙相关蛋白的保守证实了这些元素是脊椎动物视觉系统的基本特征。另一方面，视网膜的代谢成分，如神经递质前体氨基酸和Müler细胞，对与迁移相关的环境变化更敏感。
Lampreys are one of the two surviving groups of the agnathan (jawless) stages in vertebrate evolution and are thus ideal candidates for elucidating the evolution of visual systems. This study investigated the retinal amino acid neurochemistry of the southern hemisphere lamprey Geotria australis during the downstream migration of the young, recently-metamorphosed juveniles to the sea and during the upstream migration of the fully-grown and sexually-maturing adults to their spawning areas. Glutamate and taurine were distributed throughout the retina, whilst GABA and glycine were confined to neurons of the inner retina matching patterns seen in most other vertebrates. Glutamine and aspartate immunoreactivity was closely matched to Müller cell morphology. Between the migratory phases, few differences were observed in the distribution of major neurotransmitters i.e. glutamate, GABA and glycine, but changes in amino acids associated with retinal metabolism i.e. glutamine and aspartate, were evident. Taurine immunoreactivity was mostly conserved between migrant stages, consistent with its role in primary cell functions such as osmoregulation. Further investigation of glutamate signalling using the probe agmatine (AGB) to map cation channel permeability revealed entry of AGB into photoreceptors and horizontal cells followed by accumulation in inner retinal neurons. Similarities in AGB profiles between upstream and downstream migrant of G. australis confirmed the conservation of glutamate neurotransmission. Finally, calcium binding proteins, calbindin and calretinin were localized to the inner retina whilst recoverin was localized to photoreceptors. Overall, conservation of major amino acid neurotransmitters and calcium-associated proteins in the lamprey retina confirms these elements as essential features of the vertebrate visual system. On the other hand, metabolic elements of the retina such as neurotransmitter precursor amino acids and Müller cells are more sensitive to environmental changes associated with migration.
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影响因子：
1.9
作者：
Collin, SP;Hart, NS;Potter, IC
通讯作者：
Potter, IC
影响因子：
2.5
作者：
DalilThiney, N;VersauxBotteri, C;NguyenLegros, J
通讯作者：
NguyenLegros, J
影响因子：
2.5
作者：
DAVANGER, S;OTTERSEN, OP;STORMMATHISEN, J
通讯作者：
STORMMATHISEN, J
DOI：
10.1007/bf00500621
发表时间：
1987-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
AGARDH, E;EHINGER, B;WU, JY
通讯作者：
WU, JY
DOI：
10.1017/s0025315400015629
发表时间：
1959-01-01
影响因子：
1.2
作者：
CARLISLE, DB;DENTON, EJ
通讯作者：
DENTON, EJ