Role of osteopontin in the process of pulpal healing following tooth replantation in mice.
Role of osteopontin in the process of pulpal healing following tooth replantation in mice.
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DOI：
10.1016/j.reth.2022.09.011
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发表时间：
2022-12
影响因子：
4.3
通讯作者：
Ohshima, Hayato
中科院分区：
文献类型：
--
作者：
Suzuki-Barrera, Kiyoko;Makishi, Sanako;Nakatomi, Mitsushiro;Saito, Kotaro;Ida-Yonemochi, Hiroko;Ohshima, Hayato
骨桥蛋白(OPN)在严重创伤后的作用尚不清楚，尤其是它与新分化的成牙本质细胞样细胞(OBLCs)分泌I型胶原(由Col1a1基因编码)的关系。在这项研究中，我们研究了OPN在修复性牙本质形成过程中的作用，重点研究了OPN基因敲除(KO)和野生型(WT)小鼠牙齿再植后的神经再支配和血管重建。将2周龄和3周龄OPN KO和WT小鼠(OPN KO 2W、OPN KO 3W、WT 2W和WT 3W组)的上颌第一磨牙分别再植，术后3~56d进行固定。在显微计算机断层扫描分析后，对脱钙样本进行Ki67、Nestin、Pgp 9.5和CD31的免疫组织化学和Col1a1的原位杂交。OPN KO3W组再植牙牙髓发生强烈炎症反应，破坏牙髓愈合，而OPN KO2W组和WT组牙髓愈合良好。术后7~14d，OPN KO3W组第三级牙本质面积较OPN KO2W组和WT组显著减少，巢蛋白阳性的新分化OBLCs比例显著降低。OPN KO3W组血管面积显著减少，牙髓愈合受阻，牙髓血运重建和神经支配失败。对于牙根发育较晚的萌出牙，在牙髓严重损伤后，OPN对于适当的神经再支配和血运重建是必要的，以沉积修复性牙本质。骨桥蛋白缺乏抑制晚期萌出牙的硬组织形成。在轻度和重度损伤中，成牙本质细胞样细胞可能来自不同的来源。骨桥蛋白对适当的神经再支配和血运重建具有重要作用。在晚期萌出牙中，骨桥蛋白是沉积修复性牙本质所必需的。
The role of osteopontin (OPN) following severe injury remains to be elucidated, especially its relationship with type I collagen (encoded by the Col1a1 gene) secretion by newly-differentiated odontoblast-like cells (OBLCs). In this study, we examined the role of OPN in the process of reparative dentin formation with a focus on reinnervation and revascularization after tooth replantation in Opn knockout (KO) and wild-type (WT) mice. Maxillary first molars of 2- and 3-week-old-Opn KO and WT mice (Opn KO 2W, Opn KO 3W, WT 2W, and WT 3W groups) were replanted, followed by fixation 3–56 days after operation. Following micro-computed tomography analysis, the decalcified samples were processed for immunohistochemistry for Ki67, Nestin, PGP 9.5, and CD31 and in situ hybridization for Col1a1. An intense inflammatory reaction occurred to disrupt pulpal healing in the replanted teeth of the Opn KO 3W group, whereas dental pulp achieved healing in the Opn KO 2W and WT groups. The tertiary dentin in the Opn KO 3W group was significantly decreased in area compared with the Opn KO 2W and WT groups, with a significantly low percentage of Nestin-positive, newly-differentiated OBLCs during postoperative days 7–14. In the Opn KO 3W group, the blood vessels were significantly decreased in area and pulp healing was disturbed with a failure of pulpal revascularization and reinnervation. OPN is necessary for proper reinnervation and revascularization to deposit reparative dentin following severe injury within the dental pulp of erupted teeth with advanced root development. Osteopontin deficiency inhibits hard tissue formation in advanced erupted teeth. Odontoblast-like cells may be different origins between mild and severe injuries. Osteopontin has an important role for proper reinnervation and revascularization. Osteopontin is necessary to deposit reparative dentin in advanced erupted teeth.
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影响因子：
4.2
作者：
Jander, S;Bussini, S;Stoll, G
通讯作者：
Stoll, G
影响因子：
5.6
作者：
Nikoloudaki G
通讯作者：
Nikoloudaki G
影响因子：
7.6
作者：
Couve, E.;Lovera, M.;Schmachtenberg, O.
通讯作者：
Schmachtenberg, O.
影响因子：
3.6
作者：
Shimizu, A;Nakakura-Ohshima, K;Ohshima, H
通讯作者：
Ohshima, H
影响因子：
2.5
作者：
Kafourou, Vasiliki;Tong, Huei Jinn;Duggal, Monty
通讯作者：
Duggal, Monty