Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance.
Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance.
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水稻 TPR 结构域 RNA 结合蛋白功能丧失赋予广谱抗病性
DOI：
10.1073/pnas.1705927115
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发表时间：
2018-03-20
影响因子：
11.1
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Zhou X;Liao H;Chern M;Yin J;Chen Y;Wang J;Zhu X;Chen Z;Yuan C;Zhao W;Wang J;Li W;He M;Ma B;Wang J;Qin P;Chen W;Wang Y;Liu J;Qian Y;Wang W;Wu X;Li P;Zhu L;Li S;Ronald PC;Chen X
携带广谱抗病基因座的重要作物为控制传染病提供了有效的策略。尽管它们很重要，但很少有人报道广谱抗病基因座，控制该性状的潜在机制在很大程度上仍不清楚。这份报告描述了一种名为“BSR-K1”的基因的鉴定，该基因具有广谱抗药性，并证明该编码蛋白调节免疫相关基因。BSR-K1在水稻中的功能丧失导致对两种严重水稻病害的广谱抗性增强，而不会对产量造成重大影响。这份报告提供了对广谱抗性的见解，并为培育耐久抗水稻提供了一种有效的策略。携带广谱抗性基因座的作物为控制传染病提供了一种有效的策略，因为这些基因座通常对同一病原菌的不同小种甚至多种病原菌产生抗性。尽管它们很重要，但只有几个作物广谱抗病基因座被报道。在这里，我们报告了水稻BSR-K1(广谱抗性Kitaake-1)突变体的鉴定和鉴定，该突变体对稻瘟病菌和水稻白叶枯病具有广谱抗性，但对主要农艺性状没有重大影响。基于图谱的克隆显示，BSR-K1编码一个含有四肽重复序列(TPRs)的蛋白，它与多个OsPAL(OsPAL1-7)基因的mRNAs结合并促进它们的周转。BSR-K1基因功能丧失导致OsPAL1-7mRNAs在BSR-K1突变体中积累。此外，OsPAL1在野生型水稻TP309中的过表达增强了对稻瘟病菌的抗性，支持了OsPAL1的作用。我们BSR-K1等位基因的发现是一个重大的概念进步，为培育广谱抗病水稻提供了有价值的工具。
Significance Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease. Despite their importance, few broad-spectrum resistance loci have been reported, and the underlying mechanisms controlling the trait remain largely unknown. This report describes the identification of a gene, called “bsr-k1,” conferring broad-spectrum resistance and demonstrates that the encoded protein regulates immunity-related genes. Loss of function of BSR-K1 in rice leads to enhanced broad-spectrum resistance to two serious rice diseases with no major penalty on yield. This report provides insights into broad-spectrum resistance and offers an efficient strategy to breeding durably resistant rice. Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1–7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1–7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1. Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.
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DOI：
10.1007/s11032-009-9323-6
发表时间：
2010-01
期刊：
Molecular breeding : new strategies in plant improvement
影响因子：
--
作者：
Pavan S;Jacobsen E;Visser RG;Bai Y
通讯作者：
Bai Y
DOI：
10.1126/science.1171652
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Panstruga R;Dodds PN
通讯作者：
Dodds PN
影响因子：
56.9
作者：
Krattinger, Simon G.;Lagudah, Evans S.;Keller, Beat
通讯作者：
Keller, Beat
拮抗受体的表观遗传调控赋予稻瘟病抗性和产量平衡。
影响因子：
56.9
作者：
Deng, Yiwen;Zhai, Keran;He, Zuhua
通讯作者：
He, Zuhua
影响因子：
7.4
作者：
Piffanelli, P;Zhou, FS;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P