Gbvdr6, a Gene Encoding a Receptor-Like Protein of Cotton (Gossypium barbadense), Confers Resistance to Verticillium Wilt in Arabidopsis and Upland Cotton.
Gbvdr6, a Gene Encoding a Receptor-Like Protein of Cotton (Gossypium barbadense), Confers Resistance to Verticillium Wilt in Arabidopsis and Upland Cotton.
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Gbvdr6 是编码棉花（海岛棉）受体样蛋白的基因，赋予拟南芥和陆地棉对黄萎病的抗性
DOI：
10.3389/fpls.2017.02272
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发表时间：
2017
影响因子：
5.6
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Yang Y;Chen T;Ling X;Ma Z
黄萎病是一种土传病害，可给棉花生产造成毁灭性的损失。由于没有有效的化学手段来防治这种疾病，控制黄萎病的唯一有效方法是通过遗传改良。因此，鉴定新的抗病基因将有助于棉花抗黄萎病的遗传改良。通过对含有部分VE同源片段的BAC文库的筛选和表达分析，从抗黄萎病棉花品种海7124中分离和克隆了大丽叶病毒诱导基因Gbvdr6。该基因位于含有Gbve1和Gbvdr5的基因簇中，与黄萎病抗性QTL热点相邻。Gbvdr6由黄萎病菌Kleb和植物激素水杨酸(SA)、茉莉酸甲酯(MeJA)和乙烯利(ETH)诱导，但不受脱落酸(ABA)诱导。Gbvdr6定位于质膜。Gbvdr6在拟南芥和棉花中的过表达增强了对大丽叶枯病的抗性。此外，JA/ET信号通路相关基因PR3、PDF1.2、ERF1和SA相关基因PR1、PR2在转基因植株中得到了结构性表达。过量表达Gbvdr6的拟南芥对MeJA的敏感性低于野生型。此外，在大丽花弧菌侵染后的早期时间点，就触发了活性氧和胼胝质的积累。这些结果表明，Gbvdr6通过调节JA/ET和SA信号通路来增强对大丽弧菌的抗性。
Verticillium wilt is a soil-borne disease that can cause devastating losses in cotton production. Because there is no effective chemical means to combat the disease, the only effective way to control Verticillium wilt is through genetic improvement. Therefore, the identification of additional disease-resistance genes will benefit efforts toward the genetic improvement of cotton resistance to Verticillium wilt. Based on screening of a BAC library with a partial Ve homologous fragment and expression analysis, a V. dahliae-induced gene, Gbvdr6, was isolated and cloned from the Verticillium wilt-resistant cotton G. barbadense cultivar Hai7124. The gene was located in the gene cluster containing Gbve1 and Gbvdr5 and adjacent to the Verticillium wilt-resistance QTL hotspot. Gbvdr6 was induced by Verticillium dahliae Kleb and by the plant hormones salicylic acid (SA), methyl jasmonate (MeJA) and ethephon (ETH) but not by abscisic acid (ABA). Gbvdr6 was localized to the plasma membrane. Overexpression of Gbvdr6 in Arabidopsis and cotton enhanced resistance to V. dahliae. Moreover, the JA/ET signaling pathway-related genes PR3, PDF 1.2, ERF1 and the SA-related genes PR1 and PR2 were constitutively expressed in transgenic plants. Gbvdr6-overexpressing Arabidopsis was less sensitive than the wild-type plant to MeJA. Furthermore, the accumulation of reactive oxygen species and callose was triggered at early time points after V. dahliae infection. These results suggest that Gbvdr6 confers resistance to V. dahliae through regulation of the JA/ET and SA signaling pathways.
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DOI：
10.4236/abb.2013.48106
发表时间：
2013-01-01
期刊：
Advances in Bioscience and Biotechnology
影响因子：
--
作者：
Blumke, A.;Somerville, S. C.;Voigt, C. A.
通讯作者：
Voigt, C. A.
影响因子：
5.3
作者：
Artico S;Nardeli SM;Brilhante O;Grossi-de-Sa MF;Alves-Ferreira M
通讯作者：
Alves-Ferreira M
影响因子：
6.9
作者：
Camacho-Cristóbal JJ;Martín-Rejano EM;Herrera-Rodríguez MB;Navarro-Gochicoa MT;Rexach J;González-Fontes A
通讯作者：
González-Fontes A
影响因子：
7.4
作者：
Fradin, Emilie F.;Zhang, Zhao;Thomma, Bart P. H. J.
通讯作者：
Thomma, Bart P. H. J.
影响因子：
11.6
作者：
Humphries, John A.;Vejlupkova, Zuzana;Smith, Laurie G.
通讯作者：
Smith, Laurie G.