Elevated electrochemical impedance in the endoluminal regions with high shear stress: implication for assessing lipid-rich atherosclerotic lesions.
Elevated electrochemical impedance in the endoluminal regions with high shear stress: implication for assessing lipid-rich atherosclerotic lesions.
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DOI：
10.1016/j.bios.2012.12.024
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发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Hsiai, Tzung K.
中科院分区：
文献类型：
--
作者：
Yu, Fei;Lee, Juhyun;Jen, Nelson;Li, Xiang;Zhang, Qian;Tang, Rui;Zhou, Qifa;Kim, Eun. S.;Hsiai, Tzung K.
在冠状动脉介入治疗过程中，识别代谢活性动脉粥样硬化病变仍然是一个未满足的临床挑战。电化学阻抗（EIS）增加响应氧化低密度脂蛋白（oxLDL）负载病变。因此，我们评估了将EIS与血管内超声（IVUS）和剪切应力（ISS）相结合是否提供了一种新的策略来评估脂肪喂养的新西兰白色（NZW）兔中oxLDL负载病变。在NZW家兔（n=8）中部署微型传热传感器以获取基线和高脂饮食（HD）后的ISS曲线。HD治疗9周后，HD组的血清oxLDL水平（mg/dL）增加了140倍，运动粘度（cP）增加了1.5倍。HD组胸主动脉的时间平均ISS（ISSave）也增加（基线：17.61±0.24 vs. 9周：25.22±0.95 dyne/cm 2，n=4），但正常饮食组保持不变（基线：22.85±0.53 dyne/cm 2 vs. 9周：22.37±0.57 dyne/cm 2，n=4）。高频血管内超声（IVUS）显示ISSave增强区域的动脉粥样硬化病变，同心双极微电极显示EIS信号升高，这与显著的抗oxLDL免疫染色相关（无oxLDL区域：497±55 Ω，n = 8 vs. oxLDL丰富病变：679±125 Ω，n = 12，P < 0.05）。无损伤和富含ox-LDL的损伤之间的组织电阻的等效电路模型进一步验证了实验EIS信号。通过应用电化学阻抗结合剪切应力和高频超声传感器，我们提供了一种新的策略来识别oxLDL负载病变。该研究证明了整合EIS、ISS和IVUS用于基于导管的方法评估机械不稳定斑块的可行性。
Identifying metabolically active atherosclerotic lesions remains an unmet clinical challenge during coronary intervention. Electrochemical impedance (EIS) increased in response to oxidized low density lipoprotein (oxLDL)-laden lesions. We hereby assessed whether integrating EIS with intravascular ultrasound (IVUS) and shear stress (ISS) provided a new strategy to assess oxLDL-laden lesions in the fat-fed New Zealand White (NZW) rabbits. A micro-heat transfer sensor was deployed to acquire the ISS profiles at baseline and post high-fat diet (HD) in the NZW rabbits (n=8). After 9 weeks of HD, serum oxLDL levels (mg/dL) increased by 140-fold, accompanied by a 1.5-fold increase in kinematic viscosity (cP) in the HD group. Time-averaged ISS (ISSave) in the thoracic aorta also increased in the HD group (baseline: 17.61±0.24 vs. 9 weeks: 25.22±0.95 dyne/cm2, n=4), but remained unchanged in the normal diet group (baseline: 22.85±0.53 dyne/cm2 vs. 9 weeks: 22.37±0.57 dyne/cm2, n=4). High-frequency Intravascular Ultrasound (IVUS) revealed atherosclerotic lesions in the regions with augmented ISSave, and concentric bipolar microelectrodes demonstrated elevated EIS signals, which were correlated with prominent anti-oxLDL immuno-staining (oxLDL-free regions: 497±55 Ω, n = 8 vs. oxLDL-rich lesions: 679±125 Ω, n = 12, P < 0.05). The equivalent circuit model for tissue resistance between the lesion-free and ox-LDL-rich lesions further validated the experimental EIS signals. By applying electrochemical impedance in conjunction with shear stress and high-frequency ultrasound sensors, we provided a new strategy to identify oxLDL-laden lesions. The study demonstrated the feasibility of integrating EIS, ISS, and IVUS for a catheter-based approach to assess mechanically unstable plaque.
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影响因子：
8.3
作者：
Giannattasio, C;Failla, M;Mancia, G
通讯作者：
Mancia, G
DOI：
10.1073/pnas.83.7.2114
发表时间：
1986-04-01
影响因子：
11.1
作者：
DAVIES, PF;REMUZZI, A;GIMBRONE, MA
通讯作者：
GIMBRONE, MA
影响因子：
4.6
作者：
Franks, W;Schenker, I;Hierlemann, A
通讯作者：
Hierlemann, A
影响因子：
3.8
作者：
Rouhanizadeh, M;Lin, TC;Hsiai, TH
通讯作者：
Hsiai, TH
影响因子：
37.8
作者：
Holvoet, P;Vanhaecke, J;Collen, D
通讯作者：
Collen, D