Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation.
Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation.
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DOI：
10.1186/s13104-022-06203-z
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发表时间：
2022-09-24
影响因子：
1.8
通讯作者：
中科院分区：
文献类型：
--
作者：
3D工程骨对于再生医学目的和骨组织精确体外模型的开发都很重要。在体外工程或开发过程中，骨组织材料刚度的变化尚未在成骨细胞矿化和骨结节形成的整个过程中得到监测。在这篇简短的研究报告中，使用原子力显微镜监测了致密胶原支架中原代成骨细胞体外骨形成过程中的刚度变化（杨氏模量）。数据分析显示，在第5天和第8天，3D骨培养物的显著硬化与矿物质沉积的发生相关（p < 0.00005）。
Engineering bone in 3D is important for both regenerative medicine purposes and for the development of accurate in vitro models of bone tissue. The changing material stiffness of bone tissue had not yet been monitored throughout the process of mineralisation and bone nodule formation by osteoblasts either during in vitro engineering or in development perspective. Within this short research note, stiffness changes (Young’s modulus) during in vitro bone formation by primary osteoblasts in dense collagen scaffolds were monitored using atomic force microscopy. Data analysis revealed significant stiffening of 3D bone cultures at day 5 and 8 that was correlated with the onset of mineral deposition (p < 0.00005).
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DOI：
10.1073/pnas.1208916109
发表时间：
2012-08-28
影响因子：
11.1
作者：
Boonrungsiman, Suwimon;Gentleman, Eileen;Stevens, Molly M.
通讯作者：
Stevens, Molly M.
影响因子：
--
作者：
Florencio-Silva R;Sasso GR;Sasso-Cerri E;Simões MJ;Cerri PS
通讯作者：
Cerri PS
影响因子：
10.2
作者：
Hassani, Ayla;Avci, Cigir Biray;Kerdar, Sajed Nazif;Amini, Hassan;Amini, Meisam;Ahmadi, Mahdi;Sakai, Shinji;Bagca, Bakiye Goker;Ozates, Neslihan Pinar;Rahbarghazi, Reza;Khoshfetrat, Ali Baradar
通讯作者：
Khoshfetrat, Ali Baradar
影响因子：
10
作者：
Kayal C;Moeendarbary E;Shipley RJ;Phillips JB
通讯作者：
Phillips JB
3D体外模型，用于研究僵硬在癌症侵袭中的作用。
影响因子：
5.8
作者：
Micalet, Auxtine;Moeendarbary, Emad;Cheema, Umber
通讯作者：
Cheema, Umber