Characterization of Treponema denticola mutants defective in the major antigenic proteins, Msp and TmpC.
Characterization of Treponema denticola mutants defective in the major antigenic proteins, Msp and TmpC.
复制标题
DOI：
10.1371/journal.pone.0113565
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yoshimura F
中科院分区：
文献类型：
--
作者：
Abiko Y;Nagano K;Yoshida Y;Yoshimura F
齿密螺旋体是一种革兰氏阴性和厌氧螺旋体，与慢性牙周炎的严重程度增加有关。在本研究中，我们确认了两种主要的抗原性蛋白是MSP和TmpC，并利用基因缺失突变体检测了它们的生理和病理作用。MSP形成一个大的复合体，定位于外膜，而TmpC以单体形式存在，主要定位于内膜。然而，TmpC也在外膜组分中检测到，但没有检测到其细胞表面暴露。MSP缺陷增加了细胞表面的疏水性和巨噬细胞样细胞分泌肿瘤坏死因子-α，而TMPC缺陷降低了自凝集和类糜蛋白酶活性。这两个突变体都与野生型一样附着在牙龈上皮细胞上，并显示出轻微的运动性降低。此外，在MSP缺陷突变体中，作为主要膜蛋白的TDE1072蛋白被取消；因此，突变体中的表型变化可以至少部分归因于TDE1072蛋白的丢失。因此，主要的抗原蛋白MSP和TmpC对齿状毛滴虫的特性，特别是细胞表面特性有显著和不同的影响。
Treponema denticola, a gram-negative and anaerobic spirochete, is associated with advancing severity of chronic periodontitis. In this study, we confirmed that two major antigenic proteinswere Msp and TmpC, and examined their physiological and pathological roles using gene-deletion mutants. Msp formed a large complex that localized to the outer membrane, while TmpC existed as a monomer and largely localized to the inner membrane. However, TmpC was also detected in the outer membrane fraction, but its cell-surface exposure was not detected. Msp defects increased cell-surface hydrophobicity and secretion of TNF-α from macrophage-like cells, whereas TmpC defects decreased autoagglutination and chymotrypsin-like protease activities. Both mutants adhered to gingival epithelial cells similarly to the wild-type and showed slightly decreased motility. In addition, in Msp-defective mutants, the TDE1072 protein, which is a major membrane protein, was abolished; therefore, phenotypic changes in the mutant can be, at least in part, attributed to the loss of the TDE1072 protein. Thus, the major antigenic proteins, Msp and TmpC, have significant and diverse impacts on the characteristics of T. denticola, especially cell surface properties.
登录
查看更多内容
影响因子：
3.7
作者：
Abiko Y;Nagano K;Yoshida Y;Yoshimura F
通讯作者：
Yoshimura F
影响因子：
2.8
作者：
Cogoni, Valentina;Morgan-Smith, Alex;Dymock, David
通讯作者：
Dymock, David
影响因子：
2.1
作者：
Fenno, JC;Wong, GWK;McBride, BC
通讯作者：
McBride, BC
影响因子：
3.2
作者：
Fenno, JC;Wong, GWK;McBride, BC
通讯作者：
McBride, BC
影响因子：
3.1
作者：
Godovikova, Valentina;Goetting-Minesky, M. Paula;Fenno, J. Christopher
通讯作者：
Fenno, J. Christopher