Dynorphin participates in interaction between depression and non-erosive reflux disease.
Dynorphin participates in interaction between depression and non-erosive reflux disease.
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DOI：
10.1007/s10388-022-00955-0
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发表时间：
2023-01
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为探讨焦虑/抑郁与NERD的关系，我们对内脏高敏感性的重要成员强啡肽（Dyn）及其相关通路进行了研究。NERD患者的Pearson相关分析和NERD大鼠模型的体内实验。第一部分：将NERD患者血清Dyn水平与临床症状及HADS评分进行Pearson相关分析。第二部分：Wistar大鼠随机分为对照组和模型组。pH值、不动时间、血清Dyn浓度、NMDAR 1和SP表达分别采用pH自动记录仪、悬尾试验、酶联免疫吸附试验、免疫组化和免疫荧光法测定。第一部分：Pearson相关分析显示，临床症状（CS）评分与HADS评分之间存在线性相关（HAD-A，HAD-D）的相关系数分别为0.385和0.273，lg（Dyn）与lg（Dyn）的相关系数分别为0.385和0.273，lg（Dyn）与lg（Dyn）的相关系数分别为0.385和0.273，lg（Dyn）与lg（Dyn）的相关系数分别为0.385和0.273。Lg（Dyn）与Lg（HAD-D评分）的相关系数r = 0.447，P = 0.002。第二部分：模型组食管下段pH值低于对照组（P < 0.01）。模型组小鼠悬尾不动时间明显长于对照组（P < 0.01）。模型组大鼠血清Dyn浓度、脊髓NMDAR 1和食管下段黏膜SP表达水平均显著高于对照组（P < 0.05）。血清强啡肽水平升高可能是抑郁症与NERD相关的一个标志。
To explore the relationships between anxiety/depression and NERD, we focused on dynorphin (Dyn), an important member of visceral hypersensitivity, and its related pathways. Pearson’s correlation analysis on patients with NERD and in vivo experiment on NERD rat model. Part 1: Pearson’s correlation analysis among serum levels of Dyn, clinical symptoms and HADS scores of NERD patients were carried on. Part 2: Wistar rats were randomly divided into 2 groups: control group and model group. The data of pH value, immobility time, serum Dyn concentration, NMDAR1 and SP expression were, respectively, derived from automatic pH recorder, tail suspension test, enzyme-linked immunosorbent assay, immunohistochemistry and immunofluorescence. Part 1: Pearson’s correlation analysis showed that there was a linear correlation between Clinical Symptom (CS) score and HADS score (HAD-A, HAD-D), and the correlation coefficients were 0.385 and 0.273 respectively; the correlation coefficient between lg (Dyn) and lg (CS score) was r = 0.441, P = 0.002; the correlation coefficient between lg(Dyn) and lg (HAD-D score) was r = 0.447, P = 0.002. Part 2: The pH value of the lower esophagus in the model group was lower than that in the control group (P < 0.01). The tail suspension immobility time of model group was significantly longer than that of control group (P < 0.01). The serum Dyn concentration and the expression level of NMDAR1 in spinal cord and SP in lower esophageal mucosa of model group were significantly higher than those of control group (P < 0.05). Increased serum dynorphin level may be a sign of correlation between depression and NERD.
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