Tat system is required for the virulence of Dickeya zeae on rice plants
Tat system is required for the virulence of Dickeya zeae on rice plants
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Dickeya zeae 对水稻的毒力需要 Tat 系统
DOI：
10.1007/s42161-018-0086-y
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发表时间：
2018-06
影响因子：
2.2
通讯作者：
刘琼光（Qiongguang Liu）
中科院分区：
文献类型：
--
作者：
Qing Zhang;Chengpeng Yu;Liping Wen;刘琼光（Qiongguang Liu）
革兰氏阴性病原菌Dickeya zeae在水稻、玉米和许多其他植物中引起软腐病。对D.玉米的毒力还不清楚。在这项研究中，我们的特点是双精氨酸易位（达特）系统的这种病原体。对D.利用自杀载体pKNG 101构建了玉米EC1基因的表达载体，并对其表型特征进行了测定。我们的结果表明，D.玉米达特系统对细菌生长无影响。△tatA、△tatB和△tatE突变体的运动能力明显降低，不能形成生物膜，而△tatC突变体的运动能力和生物膜形成能力没有明显降低。与野生型EC 1相比，△tatA、△tatB、△tatC和△tatE突变体中蛋白酶、果胶酸裂解酶和纤维素酶的产量减少，而达特突变体中毒素的产量减少。达特突变体△tatA、△tatB、△tatC和△tatE能引起马铃薯和大白菜的软腐病和烟草的过敏反应，与野生型EC 1差异不显著。而△tatA、△tatB和△tatE对水稻种子萌发的抑制作用均不显著。此外，达特突变体接种水稻后，其发病率显著降低。这揭示了达特系统在D.在水稻、马铃薯和甘蓝中的致病力是玉米D.水稻中的玉米。
The Gram-negative pathogenic bacterium Dickeya zeae causes soft rot in rice, corn and many other plants. The molecular mechanisms underlying D. zeae virulence are not clearly understood. In this study, we characterized the twin-arginine translocation (Tat) system of this pathogen. The mutants of tatA, tatB, tatC and tatE of D. zeae EC1 were generated through the use of the suicide vector pKNG101 and their phenotypic characters were determined. Our results showed that mutations in D. zeae Tat system have no effect on the bacterial growth. △tatA, △tatB and △tatE mutants reduced the motility distinctly, and could not form biofilm, while the △tatC mutant did not show a distinctive decrease on the motility and biofilm formation. Less production of proteases, pectate lyases and cellulases were shown in △tatA, △tatB, △tatC and △tatE mutants as compared with the wild type EC1, whereas the toxin in Tat mutants was reduced, as compared with the wild type. The Tat mutants △tatA, △tatB, △tatC and △tatE could cause soft rot in potato and Chinese cabbage and hypersensitive reactions (HR) in tobacco, that were not different significantly from those of the wild type EC1. However, △tatA, △tatB and △tatE were not significantly effective in the inhibition of rice seed germination compared to the wild type. Furthermore, the disease incidence of the Tat mutants decreased significantly when they were inoculated in rice plants. This reveals that the Tat system has a different role in the virulence of D. zeae in rice, potato and cabbage and it is required for the virulence of D. zeae in rice plants.
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影响因子：
4.2
作者：
Liang Y;Gao H;Chen J;Dong Y;Wu L;He Z;Liu X;Qiu G;Zhou J
通讯作者：
Zhou J
影响因子：
3.5
作者：
Caldelari, I;Mann, S;Palmer, T
通讯作者：
Palmer, T
影响因子：
3.1
作者：
Lavander, M;Ericsson, SK;Forsberg, Å
通讯作者：
Forsberg, Å
影响因子：
--
作者：
Lifang Zou;Yu-rong Li;Gongyou Chen
通讯作者：
Lifang Zou;Yu-rong Li;Gongyou Chen
DOI：
10.1007/978-1-60327-412-8_1
发表时间：
2010-01-01
期刊：
PROTEIN SECRETION: METHODS AND PROTOCOLS
影响因子：
--
作者：
Holland, I. Barry
通讯作者：
Holland, I. Barry