Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis.
Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis.
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利用 RNAseq 技术分析大肠杆菌引起的乳腺炎奶牛外周血 miRNA 的表达谱
DOI：
10.1038/s41598-018-30518-2
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发表时间：
2018-08-23
影响因子：
4.6
通讯作者：
Zan LS
中科院分区：
文献类型：
--
作者：
Luoreng ZM;Wang XP;Mei CG;Zan LS
E.大肠杆菌是奶牛乳房炎的主要病原体，但其发生发展的分子调控机制尚未完全阐明。在本研究中，E.建立大肠杆菌诱导的乳腺炎模型，利用RNASeq技术检测感染后不同时间（0、1、3、5、7 dpi）的miRNA表达谱，并筛选差异表达的miRNA。结果显示，共检测到2416个miRNAs，包括628个已知的miRNAs和1788个新发现的miRNAs。在不同时间点共发现200个差异表达的miRNAs。生物信息学分析表明，这些差异表达的miRNAs可能通过7条信号转导途径调控奶牛乳房炎的发生和发展，分别为：腺嘌呤-细胞因子受体相互作用、MAPK信号通路、趋化因子信号通路、白细胞跨内皮迁移、T细胞受体信号通路、Toll样受体信号通路和细胞粘附分子。此外，bta-miR-200 a、bta-miR-205、bta-miR-122、bta-miR-182和新发现的保守序列15_7229可能参与了大肠杆菌晚期的免疫过程。大肠杆菌引起的乳腺炎本研究结果为乳腺炎的分子网络分析和奶牛的分子育种奠定了基础。
E. coli is the main causative agent of mastitis in dairy cows, but the mechanism of molecular regulation underlying the occurrence and development of mastitis has not yet been fully elucidated. In this study, an E. coli-induced mastitis model was created and RNASeq technology was used to measure the miRNA expression profiles at different times post-infection (0, 1, 3, 5, 7 dpi), as well as to screen for differentially expressed miRNA. The results show detection of 2416 miRNAs, including 628 known miRNAs and 1788 newly discovered miRNAs. A total of 200 differentially expressed miRNAs were found at different time points. Bioinformatics analysis showed that these differentially expressed miRNAs may regulate the occurrence and development of mastitis in dairy cows through seven signal transduction pathways, namely cytokine-cytokine receptor interaction, MAPK signaling pathway, chemokine signaling pathway, leukocyte transendothelial migration, T cell receptor signaling pathway, Toll-like receptor signaling pathway, and cell adhesion molecules. In addition, bta-miR-200a, bta-miR-205, bta-miR-122, bta-miR-182 and the newly discovered conservative_15_7229 might be involved in immune process in late stage of E. coli-induced mastitis. The results of this study lay the foundation for molecular network analysis of mastitis and molecular breeding of dairy cows.
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影响因子：
4.4
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
通讯作者：
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影响因子：
5.6
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影响因子：
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作者：
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