Genomic Characterization of Aureimonas altamirensis C2P003-A Specific Member of the Microbiome of Fraxinus excelsior Trees Tolerant to Ash Dieback.
Genomic Characterization of Aureimonas altamirensis C2P003-A Specific Member of the Microbiome of Fraxinus excelsior Trees Tolerant to Ash Dieback.
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DOI：
10.3390/plants11243487
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发表时间：
2022-12-13
期刊：
影响因子：
--
通讯作者：
Ulrich A
中科院分区：
文献类型：
--
作者：
Becker R;Ulrich K;Behrendt U;Schneck V;Ulrich A
一些欧洲白蜡树对入侵病原菌漆包树（Hymenoscyphus fraxineus）引起的枯死病表现出耐受性。这些树的微生物群含有一系列特定的细菌群。通过基因组分析和植物接种试验，对其中一个属altamirensis的群进行了详细的研究。通过16S rRNA分析和系统基因组学分析，该菌株群在系统发育上与临床分离株不同。与其他研究充分的菌株相比，对具有代表性的菌株C2P003的基因组分析产生了大量独特的基因序列。基因组的功能分析揭示了C2P003与外多糖合成、蛋白质分泌和生物膜生产以及逆境适应基因相关的特征，表明C2P003能够有效地定殖灰树叶片。接种C2P003对灰树感染后幼苗的植株健康有显著的正向影响。这种效果持续了三年，并伴随着接种一年后细菌微生物组组成的显著变化。综上所述，C2P003可能通过定殖抗性或通过影响微生物组间接抑制白蜡树叶片内或叶片上的白蜡树弧菌。
Some European ash trees show tolerance towards dieback caused by the invasive pathogen Hymenoscyphus fraxineus. The microbiome of these trees harbours a range of specific bacterial groups. One of these groups belonging to the species Aureimonas altamirensis was studied in detail by genome analysis and a plant inoculation trial. The strain group was shown to be phylogenetically distinct from clinical isolates by 16S rRNA analysis and phylogenomics. Genome analysis of a representative strain C2P003 resulted in a large number of unique gene sequences in comparison to other well-studied strains of the species. A functional analysis of the genome revealed features associated with the synthesis of exopolysaccharides, protein secretion and biofilm production as well as genes for stress adaptation, suggesting the ability of C2P003 to effectively colonize ash leaves. The inoculation of ash seedlings with C2P003 showed a significant positive effect on the plant health of the seedlings that were exposed to H. fraxineus infection. This effect was maintained over a period of three years and was accompanied by a significant shift in the bacterial microbiome composition one year after inoculation. Overall, the results indicate that C2P003 may suppress H. fraxineus in or on ash leaves via colonization resistance or indirectly by affecting the microbiome.
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