Methylobacterium-induced endophyte community changes correspond with protection of plants against pathogen attack.
Methylobacterium-induced endophyte community changes correspond with protection of plants against pathogen attack.
复制标题
DOI：
10.1371/journal.pone.0046802
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Pirttilä AM
中科院分区：
文献类型：
--
作者：
Ardanov P;Sessitsch A;Häggman H;Kozyrovska N;Pirttilä AM
用通常生活在植物内部而不伤害宿主的内生细菌接种植物是一种非常有前途的生物病害控制方法。对有益细菌诱导耐药性的机制知之甚少，因为途径仅部分已知，并且通常看不到全身反应。内生细菌的群落结构会因外界因素如接种等而发生变化，并且内生细菌对病原菌表现出直接或间接的拮抗作用。早些时候，我们表明，抗性诱导的内生甲基杆菌属。在马铃薯对黑腐果胶杆菌是依赖于接种的密度，而细菌本身没有拮抗活性。为了阐明先天内生菌群落在植物响应中的作用，我们研究了在体外和温室实验中使用植物，内生菌接种剂和病原体的各种组合的群落变化。对几种马铃薯（Solanum tuberosum L.）IMBG290对病原菌黑腐假单胞菌、致病疫霉和葡萄假单胞菌致病变种的抗性。番茄DC 3000和松树（Pinus sylvestris L.）由M. extroquens DSM 13060对冷杉小格线孢Gremmeniella abietina.接种内生甲基杆菌的能力。诱导抗性的菌株依赖于植物品种、病原菌和甲基杆菌属的密度。接种物。内生菌群落组成的变化响应接种在地上部组织和相关的抗病性或易感性的疾病。我们的研究结果表明，内生甲基杆菌属。菌株对植物抗病性具有不同的影响，这可以通过宿主的内生菌群落来调节。
Plant inoculation with endophytic bacteria that normally live inside the plant without harming the host is a highly promising approach for biological disease control. The mechanism of resistance induction by beneficial bacteria is poorly understood, because pathways are only partly known and systemic responses are typically not seen. The innate endophytic community structures change in response to external factors such as inoculation, and bacterial endophytes can exhibit direct or indirect antagonism towards pathogens. Earlier we showed that resistance induction by an endophytic Methylobacterium sp. in potato towards Pectobacterium atrosepticum was dependent on the density of the inoculum, whereas the bacterium itself had no antagonistic activity. To elucidate the role of innate endophyte communities in plant responses, we studied community changes in both in vitro and greenhouse experiments using various combinations of plants, endophyte inoculants, and pathogens. Induction of resistance was studied in several potato (Solanum tuberosum L.) cultivars by Methylobacterium sp. IMBG290 against the pathogens P. atrosepticum, Phytophthora infestans and Pseudomonas syringae pv. tomato DC3000, and in pine (Pinus sylvestris L.) by M. extorquens DSM13060 against Gremmeniella abietina. The capacities of the inoculated endophytic Methylobacterium spp. strains to induce resistance were dependent on the plant cultivar, pathogen, and on the density of Methylobacterium spp. inoculum. Composition of the endophyte community changed in response to inoculation in shoot tissues and correlated with resistance or susceptibility to the disease. Our results demonstrate that endophytic Methylobacterium spp. strains have varying effects on plant disease resistance, which can be modulated through the endophyte community of the host.
登录
查看更多内容
影响因子：
2.8
作者：
Andreote, Fernando D.;Lacava, Paulo T.;Azevedo, Joao Lucio
通讯作者：
Azevedo, Joao Lucio
影响因子：
4.4
作者：
Pirttilä, AM;Laukkanen, H;Hohtola, A
通讯作者：
Hohtola, A
影响因子：
4.4
作者：
Araújo, WL;Marcon, J;Azevedo, JL
通讯作者：
Azevedo, JL
影响因子：
2.7
作者：
García-Limones, C;Hervás, A;Tena, M
通讯作者：
Tena, M
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F