Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model.
Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model.
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DOI：
10.1016/j.dental.2013.11.004
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发表时间：
2014-02
期刊：
影响因子：
5
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Li, Fang;Weir, Michael D.;Fouad, Ashraf F.;Xu, Hockin H. K.
关键词：
抗菌底漆和粘结剂有望抑制生物膜和龋齿。由于活体修复体暴露在唾液中，一个令人担忧的问题是唾液膜导致的抗菌活性减弱。本研究的目的是首次研究唾液膜在含有新型单体甲基丙烯酸二甲氨基十二烷基酯(DMADDM)或纳米银(NAG)的粘合剂中对生物被膜的影响。合成了DMADDM和NAG，并将其加入到苏格兰邦德多功能胶粘剂和底漆中。标本要么被唾液膜覆盖，要么没有被覆盖。使用来自10名捐赠者的混合唾液的微宇宙生物膜模型。使用两种培养液：人工唾液培养液(McBain)和不含唾液蛋白的脑心输液(BHI)培养液。测定菌斑微宇宙生物膜的代谢活性、菌落形成单位(CFU)和乳酸产量(n=6)。含有DMADDM和NAG的粘合剂极大地抑制了生物膜的活性，甚至对唾液膜也是如此。当使用BHI时，唾液膜在树脂表面的预涂覆显著降低了抗菌效果(p<；0.05)。当使用人工唾液介质时，在树脂上预涂唾液膜不会降低抗菌效果。这些结果表明，人工唾液在树脂表面产生了中等来源的膜，这对生物膜提供了类似于唾液膜的衰减效果。与商业对照相比，含有DMADDM的粘结剂使生物膜CFU减少了约两个数量级。新型含DMADDM和NAG的粘结剂显著减少了生物膜的生长，即使表面有唾液膜涂层，这表明在唾液丰富的环境中有很好的应用前景。DMADDM和NAG可能在广泛的底漆、粘合剂和其他修复剂中有用，以实现抗菌和防龋性。
Antibacterial primer and adhesive are promising to inhibit biofilms and caries. Since restorations in vivo are exposed to saliva, one concern is the attenuation of antibacterial activity due to salivary pellicles. The objective of this study was to investigate the effects of salivary pellicles on bonding agents containing a new monomer dimethylaminododecyl methacrylate (DMADDM) or nanoparticles of silver (NAg) against biofilms for the first time. DMADDM and NAg were synthesized and incorporated into Scotchbond Multi-Purpose adhesive and primer. Specimens were either coated or not coated with salivary pellicles. A microcosm biofilm model was used with mixed saliva from ten donors. Two types of culture medium were used: an artificial saliva medium (McBain), and Brain Heart Infusion (BHI) medium without salivary proteins. Metabolic activity, colony-forming units (CFU), and lactic acid production of plaque microcosm biofilms were measured (n = 6). Bonding agents containing DMADDM and NAg greatly inhibited biofilm activities, even with salivary pellicles. When using BHI, the pre-coating of salivary pellicles on resin surfaces significantly decreased the antibacterial effect (p < 0.05). When using artificial saliva medium, pre-coating of salivary pellicles on resin did not decrease the antibacterial effect. These results suggest that artificial saliva yielded medium-derived pellicles on resin surfaces, which provided attenuating effects on biofilms similar to salivary pellicles. Compared with the commercial control, the DMADDM-containing bonding agent reduced biofilm CFU by about two orders of magnitude. Novel DMADDM- and NAg-containing bonding agents substantially reduced biofilm growth even with salivary pellicle coating on surfaces, indicating a promising usage in saliva-rich environment. DMADDM and NAg may be useful in a wide range of primers, adhesives and other restoratives to achieve antibacterial and anti-caries capabilities.
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影响因子：
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作者：
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通讯作者：
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