Detection of pancreatic carcinomas by imaging lactose-binding protein expression in peritumoral pancreas using [18F]fluoroethyl-deoxylactose PET/CT.
Detection of pancreatic carcinomas by imaging lactose-binding protein expression in peritumoral pancreas using [18F]fluoroethyl-deoxylactose PET/CT.
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DOI：
10.1371/journal.pone.0007977
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发表时间：
2009-11-24
期刊：
影响因子：
3.7
通讯作者：
Gelovani JG
中科院分区：
文献类型：
--
作者：
Flores LG;Bertolini S;Yeh HH;Young D;Mukhopadhyay U;Pal A;Ying Y;Volgin A;Shavrin A;Soghomonyan S;Tong W;Bornmann W;Alauddin MM;Logsdon C;Gelovani JG
通过高灵敏度的诊断成像方法对胰腺癌进行早期诊断，可以挽救成千上万患者的生命，因为早期发现可以提高可切除性和生存率。目前的非侵入性诊断成像技术的分辨率和灵敏度不足的检测小尺寸（102 -3毫米）的早期胰腺癌病变。因此，我们评估了使用β-O-D-吡喃半乳糖基-（1，4 ′）-2′-脱氧-2 ′-[18 F]氟乙基-D-吡喃葡萄糖（[18 F]FEDL）进行正电子发射断层扫描和计算机断层扫描（PET/CT）成像检测小鼠中小于3 mm的L3.6pl胰腺癌原位异种移植物的有效性。[18 F]FEDL是肝癌-肠-胰腺/胰腺炎相关蛋白（HIP/PAP）的新型放射性配体，其在肿瘤周围胰腺腺泡细胞中过表达。动态PET/CT成像显示[18 F]FEDL在瘤周胰腺组织中快速积聚（4.04±2.06%ID/g），血液清除呈双指数，半衰期为1.65±0.50分钟和14.14±3.60分钟，并从其他器官和组织中快速消除，主要通过肾脏清除。使用动态PET数据的模型无关图形分析，[18 F]FEDL在肿瘤周围胰腺组织中的平均分布体积比（DVR）估计为3.57±0.60，在假手术对照胰腺中为0.94±0.72。定量放射自显影图像和光密度测定的化学染色和共配准的相邻组织切片的比较分析表明，在相应区域的[18 F]FEDL结合和HIP/PAP表达的幅度之间有很强的线性相关性（r = 0.88）。 原位分析表明，至少2-4倍的表观病变大小放大，实现了亚毫米肿瘤和近一半的小鼠胰腺肿瘤大于3毫米。我们已经证明了早期胰腺肿瘤的检测与肿瘤生物标志物HIP/PAP过表达的肿瘤周围胰腺组织的[18 F]FEDL PET/CT的非侵入性成像的可行性。[18 F]FEDL PET/CT显像对早期胰腺癌的无创性无创性检测有助于指导活检和其他成像程序，促进可切除性并改善总体预后。
Early diagnosis of pancreatic carcinoma with highly sensitive diagnostic imaging methods could save lives of many thousands of patients, because early detection increases resectability and survival rates. Current non-invasive diagnostic imaging techniques have inadequate resolution and sensitivity for detection of small size (∼2–3 mm) early pancreatic carcinoma lesions. Therefore, we have assessed the efficacy of positron emission tomography and computer tomography (PET/CT) imaging with β-O-D-galactopyranosyl-(1,4′)-2′-deoxy-2′-[18F]fluoroethyl-D-glucopyranose ([18F]FEDL) for detection of less than 3 mm orthotopic xenografts of L3.6pl pancreatic carcinomas in mice. [18F]FEDL is a novel radioligand of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP), which is overexpressed in peritumoral pancreatic acinar cells. Dynamic PET/CT imaging demonstrated rapid accumulation of [18F]FEDL in peritumoral pancreatic tissue (4.04±2.06%ID/g), bi-exponential blood clearance with half-lives of 1.65±0.50 min and 14.14±3.60 min, and rapid elimination from other organs and tissues, predominantly by renal clearance. Using model-independent graphical analysis of dynamic PET data, the average distribution volume ratio (DVR) for [18F]FEDL in peritumoral pancreatic tissue was estimated as 3.57±0.60 and 0.94±0.72 in sham-operated control pancreas. Comparative analysis of quantitative autoradiographic images and densitometry of immunohistochemically stained and co-registered adjacent tissue sections demonstrated a strong linear correlation between the magnitude of [18F]FEDL binding and HIP/PAP expression in corresponding regions (r = 0.88). The in situ analysis demonstrated that at least a 2–4 fold apparent lesion size amplification was achieved for submillimeter tumors and to nearly half a murine pancreas for tumors larger than 3 mm. We have demonstrated the feasibility of detection of early pancreatic tumors by non-invasive imaging with [18F]FEDL PET/CT of tumor biomarker HIP/PAP over-expressed in peritumoral pancreatic tissue. Non-invasive non-invasive detection of early pancreatic carcinomas with [18F]FEDL PET/CT imaging should aid the guidance of biopsies and additional imaging procedures, facilitate the resectability and improve the overall prognosis.
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影响因子：
3.3
作者：
Hervieu, Valerie;Christa, Laurence;Scoazec, Jean-Yves
通讯作者：
Scoazec, Jean-Yves
影响因子：
11.5
作者：
Ohuchida, K;Mizumoto, K;Tanaka, M
通讯作者：
Tanaka, M
DOI：
10.1093/jnci/89.6.442
发表时间：
1997-03-19
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Lowenfels, AB;Maisonneuve, P;Elias, E
通讯作者：
Elias, E
影响因子：
29.4
作者：
Giardiello, FM;Brensinger, JD;Offerhaus, JA
通讯作者：
Offerhaus, JA
影响因子：
3.5
作者：
CHRISTA, L;FELIN, M;SEVE, AP
通讯作者：
SEVE, AP