Visualizing real-time influenza virus infection, transmission and protection in ferrets.
Visualizing real-time influenza virus infection, transmission and protection in ferrets.
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DOI：
10.1038/ncomms7378
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发表时间：
2015-03-06
影响因子：
16.6
通讯作者：
Schultz-Cherry, Stacey
中科院分区：
文献类型：
--
作者：
Karlsson, Erik A.;Meliopoulos, Victoria A.;Savage, Chandra;Livingston, Brandi;Mehle, Andrew;Schultz-Cherry, Stacey
流感在雪貂中的传播效率对风险评估研究至关重要。然而，无法实时监测病毒感染和传播动态，只能提供对传播性的一瞥。在这里，我们利用一种具有复制能力的流感报告病毒来研究雪貂感染/传播的动力学。编码NanoLuc （NLuc）荧光素酶的A/California/04/2009 H1N1病毒（CA/09）感染的雪貂的生物发光成像提供了流感感染/传播的第一个实时快照。在呼吸道和特征不太明确的肺外部位观察到发光，并用成像识别动物感染，否则传统方法可能会错过。最后，报告病毒显著提高了病毒学和血清学检测的速度和灵敏度。因此，流感感染的生物发光成像可以快速确定宿主内传播、宿主间传播和病毒载量，揭示病毒的感染动态和大流行潜力。这些结果对抗病毒药物敏感性、疫苗有效性、传播性和致病性的研究具有重要意义。雪貂是研究流感传播的主要动物模型。在这里，作者使用复制能力流感报告病毒和实时生物发光成像研究了雪貂感染和传播的动力学。
Influenza transmission efficiency in ferrets is vital for risk-assessment studies. However, the inability to monitor viral infection and transmission dynamics in real time only provides a glimpse into transmissibility. Here we exploit a replication-competent influenza reporter virus to investigate dynamics of infection/transmission in ferrets. Bioluminescent imaging of ferrets infected with A/California/04/2009 H1N1 virus (CA/09) encoding NanoLuc (NLuc) luciferase provides the first real-time snapshot of influenza infection/transmission. Luminescence in the respiratory tract and in less well-characterized extra-pulmonary sites is observed, and imaging identifies infections in animals that would have otherwise been missed by traditional methods. Finally, the reporter virus significantly increases the speed and sensitivity of virological and serological assays. Thus, bioluminescent imaging of influenza infections rapidly determines intra-host dissemination, inter-host transmission and viral load, revealing infection dynamics and pandemic potential of the virus. These results have important implications for antiviral drug susceptibility, vaccine efficacy, transmissibility and pathogenicity studies. Ferrets are the main animal model used for research on influenza transmission. Here, the authors investigate the dynamics of infection and transmission in ferrets using a replication-competent influenza reporter virus and real-time bioluminescence imaging.
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影响因子：
8.2
作者：
Liu, Qiang;Liu, Dong-ying;Yang, Zhan-qiu
通讯作者：
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影响因子：
6.7
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4.3
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影响因子：
2
作者：
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通讯作者：
Vorum, Henrik