Isolation of polymorphic microsatellite loci from the Chinese alligator (Alligator sinensis)

Isolation of polymorphic microsatellite loci from the Chinese alligator (Alligator sinensis)
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扬子鳄多态性微卫星位点的分离

DOI:
10.1111/j.1755-0998.2008.02359.x
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发表时间:
2009-05
影响因子:
7.7
通讯作者:
YULI
YULI
中科院分区:
生物学1区
文献类型:
--
作者:
HAITAO ZHU, XIAOBING WU, HUI XUE, LEI WEI;YULI

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利用扬子鳄部分基因组DNA文库和富集技术,从扬子鳄群体中分离到10个微卫星位点。从888个阳性克隆中筛选出50个微卫星重复序列,其中10个具有多态性。这些微卫星位点是又一组新的A.中华绒螯蟹的亲缘关系分析和群体遗传学研究都是可行的。
Ten microsatellite loci were isolated from the Chinese alligator (Alligator sinensis), using a partial genomic DNA library and an enrichment method. Fifty microsatellite repeats were screened from the library of 888 positive clones, 10 of which were polymorphic. These microsatellite loci developed here are another set of novel molecular markers for A. sinensis, and they will be suitable for population genetic studies as well as for kinship analysis.
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