Regulation of dendritic cell migration to the draining lymph node: impact on T lymphocyte traffic and priming.
Regulation of dendritic cell migration to the draining lymph node: impact on T lymphocyte traffic and priming.
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DOI：
10.1084/jem.20030448
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发表时间：
2003-08-18
期刊：
影响因子：
--
通讯作者：
Sallusto F
中科院分区：
文献类型：
--
作者：
MartIn-Fontecha A;Sebastiani S;Höpken UE;Uguccioni M;Lipp M;Lanzavecchia A;Sallusto F
抗原致敏的树突状细胞（DC）被用作疫苗接种的天然佐剂，但影响这种治疗效果的因素知之甚少。我们研究了影响皮下注射的小鼠成熟DC向引流淋巴结迁移的参数。我们发现DC迁移的效率随注射DC的数量而变化，并且迁移到引流淋巴结的CCR 7 +/+ DC，而不是未能这样做的CCR 7 −/− DC，有效地诱导淋巴结细胞结构的快速增加，这在T细胞增殖开始之前观察到。我们还报道，通过预先注射炎性细胞因子，增加淋巴管内皮细胞中CCR 7配体CCL 21的表达，DC迁移可以增加10倍。CD 4 + T细胞反应的幅度和质量与到达淋巴结的携带抗原的DC的数量成正比，并且可以通过预注射肿瘤坏死因子来增强高达40倍，所述肿瘤坏死因子调节组织以增加DC迁移。这些结果表明，DC数量和组织炎症是基于DC的疫苗接种的关键参数。
Antigen-pulsed dendritic cells (DCs) are used as natural adjuvants for vaccination, but the factors that influence the efficacy of this treatment are poorly understood. We investigated the parameters that affect the migration of subcutaneously injected mouse-mature DCs to the draining lymph node. We found that the efficiency of DC migration varied with the number of injected DCs and that CCR7+/+ DCs migrating to the draining lymph node, but not CCR7−/− DCs that failed to do so, efficiently induced a rapid increase in lymph node cellularity, which was observed before the onset of T cell proliferation. We also report that DC migration could be increased up to 10-fold by preinjection of inflammatory cytokines that increased the expression of the CCR7 ligand CCL21 in lymphatic endothelial cells. The magnitude and quality of CD4+ T cell response was proportional to the number of antigen-carrying DCs that reached the lymph node and could be boosted up to 40-fold by preinjection of tumor necrosis factor that conditioned the tissue for increased DC migration. These results indicate that DC number and tissue inflammation are critical parameters for DC-based vaccination.
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影响因子：
15.3
作者：
Bonifaz, L;Bonnyay, D;Mahnke, K;Rivera, M;Nussenzweig, MC;Steinman, RM
通讯作者：
Steinman, RM
影响因子：
15.3
作者：
Cahill, R N;Frost, H;Trnka, Z
通讯作者：
Trnka, Z
影响因子：
15.3
作者：
Dieu, M C;Vanbervliet, B;Vicari, A;Bridon, J M;Oldham, E;Ait-Yahia, S;Briere, F;Zlotnik, A;Lebecque, S;Caux, C
通讯作者：
Caux, C
影响因子：
15.9
作者：
Mazzucchelli, L;Blaser, A;Uguccioni, M
通讯作者：
Uguccioni, M
影响因子：
64.5
作者：
Förster, R;Schubel, A;Lipp, M
通讯作者：
Lipp, M