Selective gene transfer to the retina using intravitreal ultrasound irradiation.
Selective gene transfer to the retina using intravitreal ultrasound irradiation.
复制标题
使用玻璃体内超声照射选择性地将基因转移到视网膜。
DOI：
10.1155/2012/412752
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发表时间：
2012
影响因子：
1.9
通讯作者：
Sakamoto T
中科院分区：
文献类型：
--
作者：
Sonoda S;Tachibana K;Yamashita T;Shirasawa M;Terasaki H;Uchino E;Suzuki R;Maruyama K;Sakamoto T
本文旨在评价玻璃体内超声（US）照射的绿色荧光蛋白（GFP）质粒转移到兔视网膜使用微型超声换能器的效率。玻璃体内超声照射是通过对经结膜无缝合玻璃体切割系统进行轻微修改，利用小探针进行的。玻璃体切除术后，通过巩膜切口插入US探头。将GFP质粒（50 μL）和气泡脂质体（BL; 50 μL）的混合物注射到玻璃体腔中，并使用SonoPore 4000对视网膜产生US。对照组不暴露于US。72小时后，通过计数GFP阳性细胞的数量来定量基因转移效率。接受质粒、BL和US的视网膜显示GFP阳性细胞的数量（平均值± SEM）显著增加（32 ± 4.9; n = 7; P < 0.01）。在对照眼（n = 7）中未观察到GFP阳性细胞。玻璃体内视网膜超声照射可以将GFP质粒转移到视网膜中而不引起任何明显的损伤。这种方法可用于将基因和药物直接转移到视网膜，因此具有潜在的治疗价值。
This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vitrectomy system utilizing a small probe. After vitrectomy, the US probe was inserted through a scleral incision. A mixture of GFP plasmid (50 μL) and bubble liposomes (BLs; 50 μL) was injected into the vitreous cavity, and US was generated to the retina using a SonoPore 4000. The control group was not exposed to US. After 72 h, the gene-transfer efficiency was quantified by counting the number of GFP-positive cells. The retinas that received plasmid, BL, and US showed a significant increase in the number (average ± SEM) of GFP-positive cells (32 ± 4.9; n = 7; P < 0.01 ). No GFP-positive cells were observed in the control eyes (n = 7). Intravitreal retinal US irradiation can transfer the GFP plasmid into the retina without causing any apparent damage. This procedure could be used to transfer genes and drugs directly to the retina and therefore has potential therapeutic value.
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影响因子：
4.4
作者：
Sonoda, S;Tachibana, K;Sakamoto, T
通讯作者：
Sakamoto, T
影响因子：
3.6
作者：
Ueno, Yoshinori;Sonoda, Shozo;Komiya, Setsuro
通讯作者：
Komiya, Setsuro
影响因子：
10.8
作者：
Mehier-Humbert, S;Bettinger, T;Guy, RH
通讯作者：
Guy, RH
影响因子：
10.8
作者：
Suzuki, Ryo;Takizawa, Tomoko;Maruyama, Kazuo
通讯作者：
Maruyama, Kazuo
影响因子：
4.5
作者：
Suzuki, Ryo;Takizawa, Tomoko;Maruyama, Kazuo
通讯作者：
Maruyama, Kazuo