Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants
Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants
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杨树 NF-YB 基因家族的全基因组分析并鉴定出 PtNF-YB1 在调节转基因植物开花时间中起重要作用
DOI：
10.1186/s12870-019-1863-2
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发表时间：
2019-06
影响因子：
5.3
通讯作者：
LingLi Li
中科院分区：
文献类型：
--
作者：
Rongkai Wang;Ling Zhu;Yi Zhang;Junfeng Fan;LingLi Li
与一年生草本植物相比，木本多年生植物从幼期到开花需要更长的时间，许多性状只有在成年期才能表达，这严重影响了新品种的育种效率。对于杨树早花的研究，主要集中在拟南芥同源基因CO/FT的研究上。基于对拟南芥、水稻等植物的研究，NF-Y亚基对开花时间的调控研究取得了一些重要进展。然而，对NF-Y调控杨树开花的功能了解甚少。在本研究中，我们在杨树中鉴定了PtNF-YB家族成员，并重点研究了PtNF-YB1在转基因拟南芥和番茄上调控开花时间的功能。为了了解这一机制，利用RT-PCR技术检测了转基因拟南芥中3个已知开花基因（CO、FT和SOC1）的表达水平。我们使用Y2H和BiFC来分析PtNF-YB1和PtCO （PtCO1和PtCO2）蛋白之间的相互作用。最后，探讨了PtNF-YB1调控杨树开花的潜在分子机制模型。在本研究中，我们对杨树NF-YB基因家族进行了鉴定，并确认了PtNF-YB1调控开花时间的功能。同时，我们发现PtNF-YB1促进早花的功能可以克服物种障碍。因此，PtNF-YB1可以作为一个潜在的候选基因，通过遗传转化促进杨树和其他作物的早花。
Compared with annual herbaceous plants, woody perennials require a longer period of juvenile phase to flowering, and many traits can be only expressed in adulthood, which seriously makes the breeding efficiency of new varieties slower. For the study of poplar early flowering, the main focus is on the study Arabidopsis homologue gene CO/FT. Based on studies of Arabidopsis, rice and other plant species, some important research progress has been made on the regulation of flowering time by NF-Y subunits. However, little is known about the function of NF-Y regulating flowering in poplar. In the present study, we have identified PtNF-YB family members in poplar and focus on the function of the PtNF-YB1 regulate flowering timing using transgenic Arabidopsis and tomato. To understand this mechanisms, the expression levels of three known flowering genes (CO, FT and SOC1) were examined with RT-PCR in transgenic Arabidopsis. We used the Y2H and BiFC to assay the interactions between PtNF-YB1 and PtCO (PtCO1 and PtCO2) proteins. Finally, the potential molecular mechanism model in which PtNF-YB1 play a role in regulating flowering in poplar was discussed. In this study, we have characterized the poplar NF-YB gene family and confirmed the function of the PtNF-YB1 regulate flowering timing. At the same time, we found that the function of PtNF-YB1 to improve early flowering can overcome species barriers. Therefore, PtNF-YB1 can be used as a potential candidate gene to improve early flowering by genetic transformation in poplar and other crops.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
10.1093/dnares/dsr040
发表时间：
2012
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Maruyama K;Todaka D;Mizoi J;Yoshida T;Kidokoro S;Matsukura S;Takasaki H;Sakurai T;Yamamoto YY;Yoshiwara K;Kojima M;Sakakibara H;Shinozaki K;Yamaguchi-Shinozaki K
通讯作者：
Yamaguchi-Shinozaki K
DOI：
10.5483/bmbrep.2007.40.6.1083
发表时间：
2007-11
期刊：
Journal of biochemistry and molecular biology
影响因子：
--
作者：
N. Chen;Xiulin Zhang;P. Wei;Qi-jun Chen;Fei Ren;Jia Chen;Xuecheng Wang
通讯作者：
N. Chen;Xiulin Zhang;P. Wei;Qi-jun Chen;Fei Ren;Jia Chen;Xuecheng Wang
影响因子：
2.9
作者：
Troy J. Stephenson;Troy J. Stephenson;C. McIntyre;C. Collet;G. Xue
通讯作者：
Troy J. Stephenson;Troy J. Stephenson;C. McIntyre;C. Collet;G. Xue
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen