Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
Genetic mapping and characteristics of genes specifically or preferentially expressed during fiber development in cotton.
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DOI：
10.1371/journal.pone.0054444
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Li X;Yuan D;Zhang J;Lin Z;Zhang X
棉纤维是研究植物细胞伸长和细胞壁构建的理想模型。在纤维发育过程中，一些基因和蛋白质已被报道特异性或优先表达。对这些基因的定位将揭示这些基因在基因组中的分布，并将有助于棉花育种中的选择。本研究在前人研究的基础上，设计了331条基因引物和164条蛋白质引物，利用单链构象多态性（SSCP）技术对它们进行了定位，并整合到我们的种间BC 1连锁图谱中。这导致了在22条染色体上代表51个基因或蛋白质的57个位点的映射。对于与数量性状基因座紧密连锁的3个标记，本研究获得的QTL功能与以往报道的基因功能一致。对52个多态性功能引物进行RT-PCR分析，结果表明，有21个基因引物和17个蛋白质引物在陆地棉品种棉兰22和3-79中存在差异表达。barbadense）。3个与QTL紧密连锁的标记的RT-PCR和qRT-PCR分析结果与QTL分析和田间试验结果一致。基因本体论（GO）分类揭示了几乎所有51个定位的基因都属于有助于纤维发育的多个类别，表明纤维发育是一个受多种基因调控的复杂过程。这51个基因都在纤维细胞伸长和次生壁生物合成过程中特异或优先表达。因此，这些与功能基因相关的标记将有利于棉花纤维长度和比强度的遗传改良。
Cotton fiber is an ideal model to study cell elongation and cell wall construction in plants. During fiber development, some genes and proteins have been reported to be specifically or preferentially expressed. Mapping of them will reveal the genomic distribution of these genes, and will facilitate selection in cotton breeding. Based on previous reports, we designed 331 gene primers and 164 protein primers, and used single-strand conformation polymorphism (SSCP) to map and integrate them into our interspecific BC1 linkage map. This resulted in the mapping of 57 loci representing 51 genes or proteins on 22 chromosomes. For those three markers which were tightly linked with quantitative trait loci (QTLs), the QTL functions obtained in this study and gene functions reported in previous reports were consistent. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of 52 polymorphic functional primers showed that 21 gene primers and 17 protein primers had differential expression between Emian22 (Gossypium hirsutum) and 3–79 (G. barbadense). Both RT-PCR and quantitative real-time PCR (qRT-PCR) analyses of the three markers tightly linked with QTLs were consistent with QTL analysis and field experiments. Gene Ontology (GO) categorization revealed that almost all 51 mapped genes belonged to multiple categories that contribute to fiber development, indicating that fiber development is a complex process regulated by various genes. These 51 genes were all specifically or preferentially expressed during fiber cell elongation and secondary wall biosynthesis. Therefore, these functional gene-related markers would be beneficial for the genetic improvement of cotton fiber length and strength.
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DOI：
10.3724/sp.j.1006.2009.01217
发表时间：
2009-01-01
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
Ju Ming, Ju Ming;Wang HaiTang, Wang HaiTang;Guo, W. Z.
通讯作者：
Guo, W. Z.
影响因子：
3.1
作者：
Li, Ximei;Yuan, Daojun;Zhang, Xianlong
通讯作者：
Zhang, Xianlong
影响因子：
2
作者：
Lin, Z;He, D;Stewart, JM
通讯作者：
Stewart, JM
影响因子：
3
作者：
Liu, Chuanxiang;Lin, Zhongxu;Zhang, Xianlong
通讯作者：
Zhang, Xianlong
影响因子：
5.4
作者：
Guo, Wangzhen;Wang, Wei;Zhang, Tianzhen
通讯作者：
Zhang, Tianzhen