Cloning whole bacterial genomes in yeast.
Cloning whole bacterial genomes in yeast.
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DOI：
10.1093/nar/gkq119
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发表时间：
2010-05
影响因子：
14.9
通讯作者：
Hutchison CA 3rd
中科院分区：
文献类型：
--
作者：
Benders GA;Noskov VN;Denisova EA;Lartigue C;Gibson DG;Assad-Garcia N;Chuang RY;Carrera W;Moodie M;Algire MA;Phan Q;Alperovich N;Vashee S;Merryman C;Venter JC;Smith HO;Glass JI;Hutchison CA 3rd
大多数微生物还没有被培养过，而且许多可培养的微生物繁殖起来困难、危险或昂贵，或者在遗传上难以处理。常规克隆这些生物的大基因组片段或全基因组将大大加强它们的发现和遗传和功能表征。在这里，我们报道了在酵母中克隆全细菌基因组为单dna分子。我们克隆了生殖支原体（0.6 Mb）、肺炎支原体（0.8 Mb）和真菌支原体亚种capri （1.1 Mb）的基因组作为酵母圆形着丝粒质粒。这些基因组似乎在宿主中稳定地维持，宿主具有有效的、完善的DNA操作方法。
Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.
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影响因子：
3.2
作者：
Dhandayuthapani, S;Blaylock, MW;Baseman, JB
通讯作者：
Baseman, JB
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
64.8
作者：
BEACH, D;PIPER, M;SHALL, S
通讯作者：
SHALL, S
影响因子：
48
作者：
Itaya, Mitsuhiro;Fujita, Kyoko;Tsuge, Kenji
通讯作者：
Tsuge, Kenji
影响因子：
64.8
作者：
GOURSOT, R;GOZE, A;EHRLICH, SD
通讯作者：
EHRLICH, SD