High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh.
High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh.
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DOI：
10.1038/s41598-020-75608-2
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发表时间：
2020-10-29
影响因子：
4.6
通讯作者：
Olsen JE
中科院分区：
文献类型：
--
作者：
Ahmed S;Das T;Islam MZ;Herrero-Fresno A;Biswas PK;Olsen JE
粘菌素是用于治疗多重耐药革兰氏阴性菌引起的人类感染的最后一种抗菌素。然而，粘菌素仍广泛用于孟加拉国的集约化家禽生产。本研究旨在研究肉鸡耐粘菌素共生大肠杆菌的动态和遗传多样性。20个肉鸡养殖场在生产期间的3个时间点共鉴定出1200株大肠杆菌。采用多重PCR技术对所有菌株进行mcr-1 ~ mcr-5基因筛选，并采用重复基因外回文(REP)-PCR指纹图谱检测其遗传多样性。通过全基因组测序（WGS）进行基因组多样性和鉴定。25%的共生大肠杆菌菌株携带mcr-1基因。mcr-1基因检测频率与禽群使用粘菌素呈正相关（优势比1.71;95% CI 0.96-3.06; p = 0.068）。REP-PCR和WGS分析显示，不同的大肠杆菌群体携带多种耐药基因。从本研究中恢复的携带mcr-1的菌株与全球菌株收集的系统发育比较显示出广泛的系统发育关系。本研究发现mcr-1基因在孟加拉国肉鸡的遗传多样性大肠杆菌群体中高度流行，这表明mcr-1的大规模水平传播而不是克隆扩增。
Colistin is a last-resort antimicrobial used for the treatment of human infections caused by multidrug-resistant Gram-negative bacteria. However, colistin is still widely used in intensive poultry production in Bangladesh. We aimed to investigate the dynamics and genetic diversity of colistin-resistant commensal Escherichia coli from broiler chickens. A total of 1200 E. coli strains were characterized from 20 broiler farms at three-time points along the production period. All strains were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured by repetitive extragenic palindromic (REP)-PCR fingerprinting. Genomic diversity and characterization were performed by whole genome sequencing (WGS). Twenty-five percent of the commensal E. coli strains harbored mcr-1 genes. Frequency of mcr-1 gene detection correlated positively (odds ratio 1.71; 95% CI 0.96–3.06; p = 0.068) with the use of colistin in poultry flocks. REP-PCR profiles and WGS analysis showed diverse E. coli population carrying multiple antimicrobial resistance genes. Phylogenetic comparison of mcr-1-bearing strains recovered from this study with a global strain collection revealed wide phylogenetic relationship. This study identified a high prevalence of mcr-1 gene among genetically diverse E. coli populations from broiler chickens in Bangladesh suggesting a massive horizontal spread of mcr-1 rather than by clonal expansion.
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DOI：
10.1093/bioinformatics/btx610
发表时间：
2018-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Hadfield J;Croucher NJ;Goater RJ;Abudahab K;Aanensen DM;Harris SR
通讯作者：
Harris SR
影响因子：
3
作者：
Heras J;Domínguez C;Mata E;Pascual V;Lozano C;Torres C;Zarazaga M
通讯作者：
Zarazaga M
影响因子：
4
作者：
Herrero-Fresno, A.;Larsen, I.;Olsen, J. E.
通讯作者：
Olsen, J. E.
影响因子：
4.5
作者：
de Been M;Lanza VF;de Toro M;Scharringa J;Dohmen W;Du Y;Hu J;Lei Y;Li N;Tooming-Klunderud A;Heederik DJ;Fluit AC;Bonten MJ;Willems RJ;de la Cruz F;van Schaik W
通讯作者：
van Schaik W
基于物种特异性 PCR 检测印度食品中的大肠杆菌
影响因子：
2.8
作者：
Godambe, Lipika Pansare;Bandekar, Jayant;Shashidhar, Ravindranath
通讯作者：
Shashidhar, Ravindranath