Improved sensitivity, accuracy and prediction provided by a high-performance liquid chromatography screen for the isolation of phytase-harbouring organisms from environmental samples.
Improved sensitivity, accuracy and prediction provided by a high-performance liquid chromatography screen for the isolation of phytase-harbouring organisms from environmental samples.
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DOI：
10.1111/1751-7915.13733
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发表时间：
2021-07
影响因子：
5.7
通讯作者：
Brearley CA
中科院分区：
文献类型：
--
作者：
Rix GD;Todd JD;Neal AL;Brearley CA
基于HPLC的土壤筛选鉴定了多种肌醇多磷酸磷酸酶作为土壤总植酸酶活性的贡献者。高效液相色谱法还揭示了不同组氨酸磷酸酶对植酸盐的攻击位置，为从其他环境中分离植酸酶提供了机会。高效液相色谱法对微生物群落和纯培养物的植酸酶活性分类具有预测价值。应用于初始筛选，它们避免了因底物杂质和依赖植酸特异性介质的方法不精确而产生的“假阳性”检测问题。在这样做的过程中，他们简化了生物技术应用候选人的选择。结合16S测序和简单的生物信息学，他们揭示了最常用于生物技术用途的组氨酸磷酸酶类植酸酶的多样性。他们揭示了多种肌醇-多磷酸磷酸酶（MINPP）活性对土壤植酸酶活性聚集的贡献，并确定不动杆菌属具有这种普遍的土壤植酸酶活性。以前，在细菌中，MINPP仅被描述为肠道共生体的活性。高效液相色谱法还以一种简单的方式鉴定了一种已知的商业上成功的组氨酸（酸）磷酸酶。所描述的方法为从其他环境中分离出用于生物技术的植酸酶提供了机会。它们揭示了不同组氨酸磷酸酶对植酸的攻击位置，这是其他方法所缺乏的。
HPLC‐based screening of soil identifies Multiple Inositol Polyphosphate Phosphatase as a contributor to aggregate soil phytase activity. HPLC also reveals the position of attack on phytate by different histidine phosphatases and affords opportunity for isolation of phytases for biotechnological use from other environments. HPLC methods are shown to be of predictive value for classification of phytase activity of aggregate microbial communities and pure cultures. Applied in initial screens, they obviate the problems of ‘false‐positive’ detection arising from impurity of substrate and imprecision of methodologies that rely on phytate‐specific media. In doing so, they simplify selection of candidates for biotechnological applications. Combined with 16S sequencing and simple bioinformatics, they reveal diversity of the histidine phosphatase class of phytases most commonly exploited for biotechnological use. They reveal contribution of multiple inositol‐polyphosphate phosphatase (MINPP) activity to aggregate soil phytase activity, and they identity Acinetobacter spp. as harbouring this prevalent soil phytase activity. Previously, among bacteria MINPP was described exclusively as an activity of gut commensals. HPLC methods have also identified, in a facile manner, a known commercially successful histidine (acid) phosphatase enzyme. The methods described afford opportunity for isolation of phytases for biotechnological use from other environments. They reveal the position of attack on phytate by diverse histidine phosphatases, something that other methods lack.
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影响因子：
3.7
作者：
da Silva, Caio Abercio;Callegari, Marco Aurelio;Hermes, Rafael
通讯作者：
Hermes, Rafael
影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
DOI：
10.1016/j.ijfoodmicro.2009.07.015
发表时间：
2009-09-30
影响因子：
5.4
作者：
Haros, Monika;Carlsson, Nils-Gunnar;Andlid, Thomas
通讯作者：
Andlid, Thomas
影响因子：
3.9
作者：
GREINER, R;KONIETZNY, U;JANY, KD
通讯作者：
JANY, KD
影响因子：
9.8
作者：
Chen, Xiaodong;Jiang, Nan;Chen, Lijun
通讯作者：
Chen, Lijun