Early osteogenic signal expression of rat bone marrow stromal cells is influenced by both hydroxyapatite nanoparticle content and initial cell seeding density in biodegradable nanocomposite scaffolds.
Early osteogenic signal expression of rat bone marrow stromal cells is influenced by both hydroxyapatite nanoparticle content and initial cell seeding density in biodegradable nanocomposite scaffolds.
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大鼠骨髓基质细胞的早期成骨信号表达受到可生物降解纳米复合材料支架中的羟基磷灰石纳米含量和初始细胞播种密度的影响。
DOI：
10.1016/j.actbio.2010.11.007
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发表时间：
2011-03
影响因子：
9.7
通讯作者：
Fisher, John P.
中科院分区：
文献类型：
--
作者：
Kim, Kyobum;Dean, David;Lu, Anqi;Mikos, Antonios G.;Fisher, John P.
关键词：
在可降解聚合物支架内掺入羟基磷灰石（HA）可以提供更接近模拟天然骨组织生理学的有利合成微环境。HA纳米颗粒的掺入和旁分泌细胞-细胞信号传导距离的改变都可能影响细胞间信号传导机制，并促进植入细胞群中成骨信号表达的增强。在这项研究中，我们调查的HA纳米粒子到聚（富马酸丙二醇酯）（PPF）的复合支架的表面性能和早期成骨生长因子基因的表达与初始细胞接种密度的影响纳入支架。表面表征结果表明，HA的加入改善了PPF/HA复合支架的表面性质，表现为粗糙度、亲水性、蛋白质吸附和初始细胞附着增加。大鼠骨髓基质细胞（BMSC），即CD 34（-），CD 45（-），CD 29（+），和CD 90（+），在3D大孔PPF/HA支架上培养8天，两种不同的初始细胞接种密度（0.33和1.00百万细胞/支架）。结果表明，内源性成骨信号表达谱，包括骨形态发生蛋白-2，成纤维细胞生长因子-2，转化生长因子-β1，以及转录因子Runx 2受到HA量和初始细胞接种密度的影响。成骨生长因子基因的上调表达与大鼠BMSCs在三维支架上的成骨分化有关，其特征在于碱性磷酸酶活性、骨钙素mRNA表达和钙沉积。因此，PPF/HA复合支架的构建参数，包括HA的掺入量和初始细胞接种密度，可以用来诱导移植的大鼠BMSCs的成骨分化。
Incorporation of hydroxyapatite (HA) within a degradable polymeric scaffold may provide a favorable synthetic microenvironment that more closely mimics natural bone tissue physiology. Both incorporation of HA nanoparticles and alteration of paracrine cell-cell signaling distances may affect the intercellular signaling mechanism and facilitate the enhanced osteogenic signal expressions among the implanted cell population. In this study, we investigate the effect of the incorporation of HA nanoparticles into poly(propylene fumarate) (PPF) scaffolds on the surface properties of composite scaffolds and early osteogenic growth factor gene expression in relation to initial cell seeding density. The result of surface characterization indicated that HA addition improved surface properties of PPF/HA composite scaffolds by showing increased roughness, hydrophilicity, protein adsorption, and initial cell attachment. Rat bone marrow stromal cells (BMSCs), which were CD34(−), CD45(−), CD29(+), and CD90(+), were cultured on 3D macroporous PPF/HA scaffolds with two different initial cell seeding densities (0.33 and 1.00 million cells per scaffold) for 8 days. Results demonstrated that endogenous osteogenic signal expression profiles, including bone morphogenetic protein-2, fibroblast growth factor-2, and transforming growth factor-β1, as well as the transcriptional factor Runx2 were affected by both HA amount and initial cell seeding density. Upregulated expression of osteogenic growth factor genes was related to subsequent osteoblastic differentiation of rat BMSCs on 3D scaffolds, as characterized by alkaline phosphatase activity, osteocalcin mRNA expression, and calcium deposition. Thus PPF/HA composite scaffold construction parameters, including incorporated HA amount and initial cell seeding density, may be utilized to induce the osteoblastic differentiation of transplanted rat BMSCs.
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影响因子：
6.2
作者：
Lee, Kee-Won;Wang, Shanfeng;Lu, Lichun
通讯作者：
Lu, Lichun
影响因子：
2.4
作者：
GIBSON, LJ
通讯作者：
GIBSON, LJ
影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
影响因子：
--
作者：
Dean, D;Wolfe, MS;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
2.5
作者：
Choi, KY;Kim, HJ;Ryoo, HM
通讯作者：
Ryoo, HM