Wnt7a induces satellite cell expansion, myofiber hyperplasia and hypertrophy in rat craniofacial muscle.
Wnt7a induces satellite cell expansion, myofiber hyperplasia and hypertrophy in rat craniofacial muscle.
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Wnt7a诱导大鼠颅面肌卫星细胞扩张、肌纤维增生和肥大
DOI：
10.1038/s41598-018-28917-6
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发表时间：
2018-07-13
影响因子：
4.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Cheng X;Huang H;Luo X;Shi B;Li J
颅面肌肉驱动着头部的关键功能，包括说话、进食和表达。与躯干和四肢的肌肉相比，颅面部肌肉具有不同的胚胎起源，这可能导致不同的生长模式和再生潜力。在这项研究中，大鼠腭帆提肌和咬肌与胫骨前肌在它们对外源性Wnt 7a刺激的反应进行了比较，这已被证明是有效的促进肌肉再生的四肢。在基础条件下和单剂量注射重组人Wnt 7a后进行组织学、细胞和分子分析。在基础状态下，腭帆提肌的卫星细胞明显多于其他肌。Wnt 7a处理后，三种肌肉均普遍出现再生相关活动，包括卫星细胞扩张、肌纤维增生和肥大，但程度有明显差异。快/慢肌纤维的组成发生了实质性的变化，并且三种肌肉之间的模式各不相同。平面细胞极性通路、Akt/mTOR通路和肌生长抑制素通路中核心组分的表达水平也观察到位置特异性改变。总之，颅面和四肢肌肉可以有效地扩大外源性Wnt 7a刺激，但肌肉的反应模式存在差异。
Craniofacial muscles drive critical functions in the head, including speech, feeding and expression. Compared with their counterparts in trunk and limbs, craniofacial muscles are of distinct embryonic origins, which might consequently lead to different growth patterns and regenerative potential. In this study, rat levator veli palatini muscle and masseter muscle were compared with tibialis anterior muscle in their response to exogenous Wnt7a stimulus, which has been proved effective in promoting muscle regeneration in the limbs. Histological, cellular and molecular analyses were performed both under basal condition and after a single dose injection of recombinant human Wnt7a. Under basal condition, levator veli palatini muscle demonstrated considerably more satellite cells than the others. After Wnt7a administration, regeneration-related activities, including satellite cell expansion, myofiber hyperplasia and hypertrophy were generally observed in all three muscles, but with obvious differences in the extent. The composition of fast/slow myofibers underwent substantial alterations, and the pattern varied among the three muscles. Location-specific alterations in the expression level of core components in planar cell polarity pathway, Akt/mTOR pathway and myostatin pathway were also observed. In conclusion, both craniofacial and limb muscles could be effectively expanded by exogenous Wnt7a stimulus, but muscle-to-muscle variations in response patterns existed.
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软pa的大鼠肌肉再生模型。
影响因子：
3.7
作者：
Carvajal Monroy PL;Grefte S;Kuijpers-Jagtman AM;Helmich MP;Ulrich DJ;Von den Hoff JW;Wagener FA
通讯作者：
Wagener FA
DOI：
10.1597/06-220.1
发表时间：
2007-09-01
期刊：
CLEFT PALATE-CRANIOFACIAL JOURNAL
影响因子：
--
作者：
Ha, Seunghee;Kuehn, David P.;Alperin, Noam
通讯作者：
Alperin, Noam
胫骨前肌和提腭肌的形态和分子比较：增强潜力的初步研究
影响因子：
2.7
作者：
Cheng X;Song L;Lan M;Shi B;Li J
通讯作者：
Li J
DOI：
10.1152/ajpregu.91020.2008
发表时间：
2009-07-01
影响因子：
2.8
作者：
Medeiros, Erika F.;Phelps, Michael P.;Bradley, Terence M.
通讯作者：
Bradley, Terence M.
影响因子：
3.7
作者：
Hardy D;Besnard A;Latil M;Jouvion G;Briand D;Thépenier C;Pascal Q;Guguin A;Gayraud-Morel B;Cavaillon JM;Tajbakhsh S;Rocheteau P;Chrétien F
通讯作者：
Chrétien F