A validated high-performance liquid chromatography method with diode array detection for simultaneous determination of nine flavonoids in Senecio cannabifolius Less.
A validated high-performance liquid chromatography method with diode array detection for simultaneous determination of nine flavonoids in Senecio cannabifolius Less.
复制标题
一种经过验证的高效液相色谱方法，采用二极管阵列检测，可同时测定千里光中的九种黄酮类化合物。
DOI：
10.1016/j.jpba.2012.12.007
复制
发表时间：
2013-03
影响因子：
3.4
通讯作者：
Li, Yu-Xin
中科院分区：
文献类型：
--
作者：
Sun, Lu-Guo;Yi, Jing-Wen;Huang, Yan-Xin;Li, Yu-Xin
建立了反相高效液相色谱-二极管阵列检测器同时测定中草药千里光中9种黄酮类成分的方法。采用Agilent Zorbax SB-C18色谱柱，流动相为乙腈-0.5%甲酸(v/v)水溶液，流速为1.0mlmin−1，流速为1.0mlmin，检测波长为360 nm。对方法的线性、精密度、检出限、日内和日间变异、重现性和回收率进行了验证。校正曲线在实验范围内线性关系良好(R2>；0.9995)。日内和日间测定的相对标准偏差(RSD)均小于3.0%。样品稳定96h以上，平均回收率在90.6%~102.5%之间。黄酮类化合物的检出限为0.028~0.085μg/ml，灵敏度高。新建立的高效液相色谱方法为大麻药材的质量保证提供了有力的手段。
A reversed phase high performance liquid chromatography method coupled with a diode array detector (HPLC-DAD) was developed for the first time for the simultaneous determination of 9 flavonoids in Senecio cannabifolius, a traditional Chinese medicinal herb. Agilent Zorbax SB-C18 column was used at room temperature and the mobile phase was a mixture of acetonitrile and 0.5% formic acid (v/v) in water in the gradient elution mode at a flow-rate of 1.0mlmin−1, detected at 360nm. Validation of this method was performed to verify the linearity, precision, limits of detection and quantification, intra- and inter-day variabilities, reproducibility and recovery. The calibration curves showed good linearities (R2>0.9995) within the test ranges. The relative standard deviation (RSD) of the method was less than 3.0% for intra- and inter-day assays. The samples were stable for at least 96h, and the average recoveries were between 90.6% and 102.5%. High sensitivity was demonstrated with detection limits of 0.028–0.085μg/ml for flavonoids. The newly established HPLC method represents a powerful technique for the quality assurance of S. cannabifolius.
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DOI：
--
发表时间：
2009
期刊：
Pharmaceutical and Clinical Research
影响因子：
--
作者：
Z. Mian
通讯作者：
Z. Mian
影响因子：
3.1
作者：
Dugo, P;Lo Presti, M;Mondello, L
通讯作者：
Mondello, L
DOI：
10.3724/sp.j.1095.2010.00061
发表时间：
2010-10
期刊：
Chinese Journal of Applied Chemistry
影响因子：
--
作者：
He Zhong-mei;Z. Ying;Xun Jia-Meng;Xiao Feng-yan
通讯作者：
He Zhong-mei;Z. Ying;Xun Jia-Meng;Xiao Feng-yan
影响因子：
3.4
作者：
Juan Su;P. Fu;Yunheng Shen;Chuan Zhang;Ming-jin Liang;Run-hui Liu;Huiliang Li;Weidong Zhang
通讯作者：
Juan Su;P. Fu;Yunheng Shen;Chuan Zhang;Ming-jin Liang;Run-hui Liu;Huiliang Li;Weidong Zhang
影响因子：
3.8
作者：
Bin Wu;W. Lin;Huiyuan Gao;Lu Zheng;Li Jun Wu;Chul-Sa Kim
通讯作者：
Bin Wu;W. Lin;Huiyuan Gao;Lu Zheng;Li Jun Wu;Chul-Sa Kim