DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1.
DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1.
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DOI：
10.3390/v9070190
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发表时间：
2017-07-18
期刊：
影响因子：
--
通讯作者：
Quax TEF
中科院分区：
文献类型：
--
作者：
Peeters E;Boon M;Rollie C;Willaert RG;Voet M;White MF;Prangishvili D;Lavigne R;Quax TEF
尽管许多细菌和真核病毒的感染周期已被详细描述，但古细菌病毒的感染周期在很大程度上仍未被探索。最近，对一些模式古细菌病毒如SIRV 2（冰岛硫化叶菌杆状病毒）的研究揭示了一种不寻常的裂解机制，该机制涉及宿主细胞表面上锥体出口结构的形成。为了扩大对SIRV 2感染周期的理解，我们的目的是功能特征gp 1，这是一个功能未知的SIRV 2基因。SIRV2_Gp1蛋白在感染的早期阶段高度表达，并且它是唯一在病毒基因组上编码两次的蛋白。它具有螺旋-转角-螺旋基序，因此被假设为结合DNA。用电泳迁移率变动分析和原子力显微镜对SIRV2_Gp1的DNA结合行为进行了表征。我们提供的证据表明，蛋白质与DNA相互作用，并形成大的聚集体，从而导致DNA的极端浓缩。此外，蛋白质的N-末端结构域介导对病毒宿主硫化叶菌的毒性。我们的研究结果可能会导致生物技术的应用，例如开发一种用于遏制病原菌的有毒肽，并增加我们对Rudiviral感染周期的理解。
Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored. Recently, studies on a few model archaeal viruses such as SIRV2 (Sulfolobus islandicus rod-shaped virus) have revealed an unusual lysis mechanism that involves the formation of pyramidal egress structures on the host cell surface. To expand understanding of the infection cycle of SIRV2, we aimed to functionally characterize gp1, which is a SIRV2 gene with unknown function. The SIRV2_Gp1 protein is highly expressed during early stages of infection and it is the only protein that is encoded twice on the viral genome. It harbours a helix-turn-helix motif and was therefore hypothesized to bind DNA. The DNA-binding behavior of SIRV2_Gp1 was characterized with electrophoretic mobility shift assays and atomic force microscopy. We provide evidence that the protein interacts with DNA and that it forms large aggregates, thereby causing extreme condensation of the DNA. Furthermore, the N-terminal domain of the protein mediates toxicity to the viral host Sulfolobus. Our findings may lead to biotechnological applications, such as the development of a toxic peptide for the containment of pathogenic bacteria, and add to our understanding of the Rudiviral infection cycle.
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影响因子：
3.9
作者：
Prangishvili D;Koonin EV;Krupovic M
通讯作者：
Krupovic M
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
5.4
作者：
Quax, Tessa E. F.;Voet, Marleen;Prangishvili, David
通讯作者：
Prangishvili, David
影响因子：
3.2
作者：
Vestergaard, Gisle;Shah, Shiraz A.;Prangishvili, David
通讯作者：
Prangishvili, David
影响因子：
5.6
作者：
Birkenbihl, RP;Neef, K;Kemper, B
通讯作者：
Kemper, B