Vacuum/compression valving (VCV) using parrafin-wax on a centrifugal microfluidic CD platform.

Vacuum/compression valving (VCV) using parrafin-wax on a centrifugal microfluidic CD platform.
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DOI:
10.1371/journal.pone.0058523
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发表时间:
2013
期刊:
影响因子:
3.7
通讯作者:
Madou M
Madou M
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Al-Faqheri W;Ibrahim F;Thio TH;Moebius J;Joseph K;Arof H;Madou M

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本文介绍了一种新型的利用石蜡的真空/压缩阀。VCV通过用蜡塞密封排气通道/孔(对于常闭阀)或用蜡密封(对于常开阀)来实现,并通过CD表面上的局部加热来激活。我们证明,VCV提供了避免在诊断过程中对样品/试剂进行不必要加热的优点,允许CD的真空密封,以及在微流体过程中石蜡与样品/试剂的清晰分离。作为一个概念的证明,液体流动切换和液体计量的微流体过程中演示的VCV。结果表明,VCV降低了所需的旋转频率,以执行具有高精度和易于控制的微流体过程。
This paper introduces novel vacuum/compression valves (VCVs) utilizing paraffin wax. A VCV is implemented by sealing the venting channel/hole with wax plugs (for normally-closed valve), or to be sealed by wax (for normally-open valve), and is activated by localized heating on the CD surface. We demonstrate that the VCV provides the advantages of avoiding unnecessary heating of the sample/reagents in the diagnostic process, allowing for vacuum sealing of the CD, and clear separation of the paraffin wax from the sample/reagents in the microfluidic process. As a proof of concept, the microfluidic processes of liquid flow switching and liquid metering is demonstrated with the VCV. Results show that the VCV lowers the required spinning frequency to perform the microfluidic processes with high accuracy and ease of control.
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