Lipid bilayers covalently anchored to carbon nanotubes.
Lipid bilayers covalently anchored to carbon nanotubes.
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DOI：
10.1021/la301094h
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发表时间：
2012-05-29
期刊：
影响因子：
--
通讯作者：
Malmstadt N
中科院分区：
文献类型：
--
作者：
Dayani Y;Malmstadt N
碳纳米管独特的物理和电学性质使其成为生物电子和生物传感等各个领域的令人兴奋的材料。由于碳纳米管的水溶性差，因此此类应用的功能化一直是一个挑战。特别需要的是用于将碳纳米管与生物分子整合并构建用于生物纳米电子应用的新型混合纳米结构的功能化方法。我们提出了一种新的方法，用于制造可分散的，生物相容的碳纳米管基材料。多壁碳纳米管（MWCNT）与伯胺轴承磷脂在马来酰亚胺活化反应共价修饰。改性后的碳纳米管在非极性溶剂中具有良好的分散性。傅里叶变换红外（FTIR）光谱显示峰归因于脂质和纳米管表面之间的酰胺键的形成。脂质修饰的纳米管与其他脂质分子的简单超声处理导致形成均匀的脂质双层包覆纳米管。这些双层包覆的纳米管在水溶液中是高度分散和稳定的。共聚焦荧光显微镜显示双层修饰的纳米管表面上的标记脂质。透射电子显微镜（TEM）显示了分散的双层包覆的多壁碳纳米管的形态。荧光淬灭的脂质涂层的多壁碳纳米管证实了双层配置的脂质在纳米管表面和荧光各向异性测量表明，该双层是流体以上的凝胶到液体的转变温度。膜蛋白α-溶血素自发插入到MWCNT支撑的双层膜中，证实了仿生膜结构。这些仿生纳米结构为碳纳米管基材料与生物分子的整合提供了一个很有前途的平台。
The unique physical and electrical properties of carbon nanotubes make them an exciting material for applications in various fields such as bioelectronics and biosensing. Due to the poor water solubility of carbon nanotubes, functionalization for such applications has been a challenge. Of particular need are functionalization methods for integrating carbon nanotubes with biomolecules and constructing novel hybrid nanostructures for bionanoelectronic applications. We present a novel method for the fabrication of dispersible, biocompatible carbon nanotube-based materials. Multi-walled carbon nanotubes (MWCNTs) are covalently modified with primary amine-bearing phospholipids in a carbodiimide-activated reaction. These modified carbon nanotubes have good dispersibility in nonpolar solvents. Fourier transform infrared (FTIR) spectroscopy shows peaks attributable to the formation of amide bonds between lipids and the nanotube surface. Simple sonication of lipid-modified nanotubes with other lipid molecules leads to the formation of a uniform lipid bilayer coating the nanotubes. These bilayer-coated nanotubes are highly dispersible and stable in aqueous solution. Confocal fluorescence microscopy shows labeled lipids on the surface of bilayer-modified nanotubes. Transmission electron microscopy (TEM) shows the morphology of dispersed bilayer-coated MWCNTs. Fluorescence quenching of lipid-coated MWCNTs confirms the bilayer configuration of the lipids on the nanotube surface and fluorescence anisotropy measurements show that the bilayer is fluid above the gel-to-liquid transition temperature. The membrane protein α-hemolysin spontaneously inserts into the MWCNT-supported bilayer, confirming the biomimetic membrane structure. These biomimetic nanostructures are a promising platform for the integration of carbon nanotube-based materials with biomolecules.
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